New Citations using Trevigen Products - October 2009

Senescence-Associated Oxidative DNA Damage Promotes the Generation of Neoplastic Cells
Karo Gosselin, Sébastien Martien, Albin Pourtier, Chantal Vercamer, Peter Ostoich, Luc Morat, Laure Sabatier, Laurence Duprez, Claire T'Kint de Roodenbeke, Eric Gilson, Nicolas Malaquin, Nicolas Wernert, Predrag Slijepcevic, Marjan Ashtari, Fazia Chelli, Emeric Deruy, Bernard Vandenbunder, Yvan De Launoit, and Corinne Abbadie
Cancer Res., Oct 2009; 69: 7917 – 7925.
...suspension was immediately laid onto a Trevigen cometslide. Agarose was allowed to solidify...and stained with either SYBR Green I (Trevigen) according to the manufacturers recommendations...oxo-7,8-dihydroguanine (8-oxoG) antibody is from Trevigen. Because it recognizes 8-oxoG in both...


Glyceraldehyde 3-Phosphate Dehydrogenase Depletion Induces Cell Cycle Arrest and Resistance to Antimetabolites in Human Carcinoma Cell Lines
Manali S. Phadke, Natalia F. Krynetskaia, Anurag K. Mishra, and Evgeny Krynetskiy
J. Pharmacol. Exp. Ther., Oct 2009; 331: 77 – 86.
...were detected by Neutral Comet assay with the CometAssay kit (Trevigen, Gaithersburg, MD), according to the manufacturer's instructions...Fragment Length Analysis using Repair Enzymes (FLARE) slides (Trevigen). After cell lysis in a neutral lysis buffer at 4C overnight...


Anti-angiogenic activity of carebastine: a plausible mechanism affecting airway remodelling
A. De Luisi, G. Mangialardi, R. Ria, G. Acuto, D. Ribatti, and A. Vacca
Eur. Respir. J., Oct 2009; 34: 958 – 966.
...24-well plates pre-coated with Cultrex basement membrane extract Trevigen, Inc., Gaithersburg, MD, USA; 400uLwell in 1mLwell of SFM...endothelial cells were seeded on Cultrex basement membrane extract Trevigen, Inc., Gaithersburg, MD, USA in: a serum-free medium SFM alone...


Udu Deficiency Activates DNA Damage Checkpoint
Chiaw-Hwee Lim, Shang-Wei Chong, and Yun-Jin Jiang
Mol. Biol. Cell, Oct 2009; 20: 4183 – 4193.
...assay was performed using the CometAssay kit (4250-040-K; Trevigen, Gaithersburg, MD) according to the manufacturer's instruction. DNA was stained with SYBR Green as provided (Trevigen). Comet images were taken by fluorescence microscopy...


Hepatitis B and Hepatitis C virus replication up-regulate serine protease inhibitor Kazal, resulting in cellular resistance to serine protease dependent apoptosis
Jason Lamontagne, Mark Pinkerton, Timothy M. Block, and Xuanyong Lu
J. Virol., Oct 2009; 10.1128/JVI.01249-09.
...BFA/CHX/Z-VAD122 7 treated cells were washed with PBS and then incubated with annexin V-FITC in 100ul of binding123 buffer (Trevigen Inc., Gaithersburg, MD). After incubating for 30 minutes, the cells were washed124 with PBS and were then visualized with a...


Proteasome-dependent Processing of Topoisomerase I-DNA Adducts into DNA Double Strand Breaks at Arrested Replication Forks
Chao-Po Lin, Yi Ban, Yi Lisa Lyu, and Leroy F. Liu
J. Biol. Chem., Oct 2009; 284: 28084 – 28092.
...streptomycin (100 mug/ml) in a 30 C incubator with 5% CO2. Neutral Comet Assay The comet assays were performed according to the Trevigen CometAssayTM kit protocol with slight modifications. Cells were pretreated with various inhibitors for 30 min, followed by...


In Utero Exposure to Di-(2-Ethylhexyl) Phthalate Decreases Mineralocorticoid Receptor Expression in the Adult Testis
D. B. Martinez-Arguelles, M. Culty, B. R. Zirkin, and V. Papadopoulos
Endocrinology, Oct 2009; 10.1210/en.2009-0847.
...difluoride membrane. The membrane was incubated with antiserum against MR (see above) and then against GAPDH (2275-PC-100; Trevigen, Gaithersburg, MD). Proteins were detected with a secondary antibody coupled to horseradish peroxidase (goat antimouse, 554002...


Effects of Sp1 overexpression on cultured human corneal stromal cells
Xiang Shen, Jeong-Seok Park, Ye Qiu, Joel Sugar, and Beatrice Y J T Yue
Genes Cells, Oct 2009; 14: 1133 - 1139.
...anti-cathepsin B, anti-a1-PI or anti-glyceraldehyde 3-phosphate dehydrogenase (Trevigen, Gaithersburg, MD, USA). Horseradish peroxidase-conjugated goat anti-rabbit IgG was used as the secondary...


Poly(Adenosine 5'-Diphosphate-Ribose) Polymerase Inhibition Counteracts Multiple Manifestations of Experimental Type 1 Diabetic Nephropathy
Viktor R. Drel, Weizheng Xu, Jie Zhang, Ivan A. Pavlov, Hanna Shevalye, Barbara Slusher, and Irina G. Obrosova
Endocrinology, Oct 2009; 10.1210/en.2009-0628.
...Mouse monoclonal anti-poly(ADP-ribose) antibody was purchased from Trevigen, Inc. (Gaithersburg, MD), rabbit poly-clonal antinitrotyrosine (NT) antibody and mouse monoclonal anti-NT antibody...


Development and Characterization of SSR Markers and Their Use to Assess Genetic Relationships among Alfalfa Germplasms
C. He, Z. L. Xia, T. A. Campbell, and G. R. Bauchan
Crop Sci., Oct 2009; 49: 2176 – 2186.
...PCR products were finally extended at 72C for 5 min. DNA fragments were sized using high-resolution 3% TREVIGEL agarose gels (Trevigen, Inc., Gaithersburg, MD) or the ABI PRISM 3100 Genetic Analyzer. Nomenclature for SSR Markers SSR names were prefixed with...


TSLP Induces Chemotactic and Pro-survival Effects in Eosinophils: Implications in Allergic Inflammation
Chun K Wong, Shuiqing Hu, Phyllis F.Y. Cheung, and Christopher WK Lam
Am. J. Respir. Cell Mol. Biol., Oct 2009; 10.1165/rcmb.2009-0168OC.
...Walkersville, MD). Page 8 of 56 9 Apoptosis assay Apoptosis of eosinophils was assessed by the TACS Annexin V-FITC assay (Trevigen, Gaithersburg, MD, USA) using flow cytometry. The population of viable...


Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells
Sajiv Chandradas, Gintaras Deikus, Jonathan G. Tardos, and Vladimir Y. Bogdanov
J. Leukoc. Biol., Oct 2009; 10.1189/jlb.0409252.
...lysates, anti-SR protein mAb 16H3. The blot was reprobed with anti-GAPDH polyclonal antibody (Trevigen, Gaithersburg, MD, USA). (B) In silico analysis of TF exon 5 using web resources ESEfinder...


Oral Poly(ADP-Ribose) Polymerase-1 Inhibitor BSI-401 Has Antitumor Activity and Synergizes with Oxaliplatin against Pancreatic Cancer, Preventing Acute Neurotoxicity
Davide Melisi, Valeria Ossovskaya, Cihui Zhu, Roberta Rosa, Jianhua Ling, Patrick M. Dougherty, Barry M. Sherman, James L. Abbruzzese, and Paul J. Chiao
Clin. Cancer Res., Oct 2009; 15: 6367 – 6377.
...activity of PARP in human pancreatic cancer cell lines and MEFs was measured using a universal colorimetric PARP assay kit (Trevigen). Apoptosis The extent of apoptosis was determined by fragmented DNA detection as previously described by Matassov...


Hepatitis C Viral Proteins induce NADPH Oxidase 4 (Nox4) in a TGF-dependent manner: A new contributor of HCV-induced oxidative stress
Howard E. Boudreau, Suzanne U. Emerson, Agnieszka Korzeniowska, Meghan A. Jendrysik, and Thomas L. Leto
J. Virol., Oct 2009; 10.1128/JVI.01059-09.
...antibody, rabbit polyclonal anti-Actin antibody, rabbit polyclonal anti-GAPDH (Trevigen, Gaithersburg, MD, USA), mouse monoclonal anti-V5, goat polyclonal anti-E2...


Acquired Resistance to Combination Treatment with Temozolomide and ABT-888 Is Mediated by Both Base Excision Repair and Homologous Recombination DNA Repair Pathways
Xuesong Liu, Edward K. Han, Mark Anderson, Yan Shi, Dimitri Semizarov, Gang Wang, Thomas McGonigal, Lisa Roberts, Loren Lasko, Joann Palma, Gui-dong Zhu, Thomas Penning, Saul Rosenberg, Vincent L. Giranda, Yan Luo, Joel Leverson, Eric F. Johnson, and Alexander R. Shoemaker
Mol. Cancer Res., Oct 2009; 7: 1686 – 1692.
...Polyclonal anti-PAR antibody was from Trevigen. Western blot analysis was done using enhanced chemiluminescence detection reagents according to the instructions...


Synergistic effects of autologous cell and hepatocyte growth factor gene therapy for neovascularization in a murine model of hindlimb ischemia
Yasutaka Yamamoto, Takashi Matsuura, Genta Narazaki, Miyoko Sugitani, Kohei Tanaka, Akihiro Maeda, Goshi Shiota, Kenzo Sato, Akio Yoshida, and Ichiro Hisatome
Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1329 - H1336.
...by terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) using a commercially available kit (TACS/XL, Trevigen, Gaithersburg, MD). Migration assay. A total of 4 105 aMNCs in 0.5 ml DMEM with 0.4% FBS were seeded in the upper chamber...


Translocator Protein Blockade Reduces Prostate Tumor Growth
Arlee Fafalios, Ardavan Akhavan, Anil V. Parwani, Robert R. Bies, Kevin J. McHugh, and Beth R. Pflug
Clin. Cancer Res., Oct 2009; 15: 6177 – 6184.
...Slides were then loaded on a Dako Autostainer. The primary anti-TSPO (working dilution, 1:350) was a polyclonal rabbit antibody (Trevigen). The immunolabeling procedures were done using Dako EnVision-Labeled Polymer-HRP antirabbit and were carried out according...


Vorinostat Inhibits Brain Metastatic Colonization in a Model of Triple-Negative Breast Cancer and Induces DNA Double-Strand Breaks
Diane Palmieri, Paul R. Lockman, Fancy C. Thomas, Emily Hua, Jeanne Herring, Elizabeth Hargrave, Matthew Johnson, Natasha Flores, Yongzhen Qian, Eleazar Vega-Valle, Kunal S. Taskar, Vinay Rudraraju, Rajendar K. Mittapalli, Julie A. Gaasch, Kaci A. Bohn, Helen R. Thorsheim, David J. Liewehr, Sean Davis, John F. Reilly, Robert Walker, Julie L. Bronder, Lionel Feigenbaum, Seth M. Steinberg, Kevin Camphausen, Paul S. Meltzer, Victoria M. Richon, Quentin R. Smith, and Patricia S. Steeg
Clin. Cancer Res., Oct 2009; 15: 6148 – 6157.
...migration assay Boyden chamber motility assays were conducted as described previously (8). Neutral comet assay kit from Trevigen was used according to the recommendations from the manufacturer. DNA was stained with propidium iodide and digital fluorescent...


A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases
Dorjbal Dorjsuren, David M. Wilson, III, William A. Beard, John P. McDonald, Christopher P. Austin, Roger Woodgate, Samuel H. Wilson, and Anton Simeonov
Nucleic Acids Res., Oct 2009; 37: e128.
...plates following previously described protocols (27). Enzymes Recombinant human DNA pol beta was either purchased from Trevigen (Gaithersburg, MD) or produced via published protocols (28). Equivalent performance was noted for the enzyme obtained...


Chelation of intracellular iron with the antifungal agent ciclopirox olamine induces cell death in leukemia and myeloma cells
Yanina Eberhard, Sean P. McDermott, Xiaoming Wang, Marcela Gronda, Amudha Venugopal, Tabitha E. Wood, Rose Hurren, Alessandro Datti, Robert A. Batey, Jeffrey Wrana, William E. Antholine, John Dick, and Aaron D. Schimmer
Blood, Oct 2009; 114: 3064 - 3073.
...Membranes were probed with polyclonal rabbit anti-human survivin (1 mug/mL), or with mouse anti-human GAPDH (Trevigen). Secondary antibodies were horseradish peroxidase-conjugated goat anti-mouse IgG (1:10 000 [vol/vol]) and...