New Citations using Trevigen Products

The Recombination Protein RAD52 Cooperates with the Excision Repair Protein OGG1 for the Repair of Oxidative Lesions in Mammalian Cells
Nadja C. de Souza-Pinto, Scott Maynard, Kazunari Hashiguchi, Jingping Hu, Meltem Muftuoglu, and Vilhelm A. Bohr
Mol. Cell. Biol., Aug 2009; 29: 4441 - 4454.
...incubated in primary anti-8-oxoG antibody (Trevigen) at a 1:250 dilution in 10 goat serum-PBS...heterochromatic DNA (7, 32, 40; see also the Trevigen web site). For the control, the results...8-oxoG antibody (Fig. 7C) that was used (Trevigen) in two ways. First, treatment of HeLa...

Poly(ADP-ribose) polymerase inhibitor ABT-888 potentiates the cytotoxic activity of temozolomide in leukemia cells: influence of mismatch repair status and O6-methylguanine-DNA methyltransferase activity
Terzah M. Horton, Gaye Jenkins, Debananda Pati, Linna Zhang, M. Eileen Dolan, Albert Ribes-Zamora, Alison A. Bertuch, Susan M. Blaney, Shannon L. Delaney, Madhuri Hegde, and Stacey L. Berg
Mol. Cancer Ther., Aug 2009; 8: 2232 - 2242.
...a commercially available PARP assay (Trevigen). Cell lysates were prepared from 1 107 leukemia cells using 1 PARP buffer (Trevigen) supplemented with 0.4 mmol/L phenylmethylsulfonyl...in triplicate, and recombinant PARP (Trevigen) was used to produce a standard curve...

Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler
Aaron J. Gottschalk, Gyula Timinszky, Stephanie E. Kong, Jingji Jin, Yong Cai, Selene K. Swanson, Michael P. Washburn, Laurence Florens, Andreas G. Ladurner, Joan W. Conaway, and Ronald C. Conaway
PNAS, Aug 2009; 106: 13770 - 13774.
...pmol) Flag-Alc1 (wild-type, E175Q, or D723A) from HEK293/FRT cells or SF9 cells, 115 ng (1 pmol) Parp1 (Trevigen), recombinant Parg (Trevigen), 34 muM nicotine adenine dinucleotide, and 150 ng mono- or oligonucleosomes from HeLa cells (37...

Differences in Immunolocalization of Kim-1, RPA-1, and RPA-2 in Kidneys of Gentamicin-, Cisplatin-, and Valproic Acid-Treated Rats: Potential Role of iNOS and Nitrotyrosine
Jun Zhang, Peter L. Goering, Parvaneh Espandiari, Martin Shaw, Joseph V. Bonventre, Vishal S. Vaidya, Ronald P. Brown, Joe Keenan, Cormac G. Kilty, Nakissa Sadrieh, and Joseph P. Hanig
Toxicol Pathol, Aug 2009; 37: 629 - 643.
...was detected using the TACS in situ apoptotic detection kit (Trevigen, Inc., Gaithersburg, MD). The TUNEL procedures were as follows...ten minutes. In the final step, uncolored soluble chromogen (Trevigen blue label) was enzymatically converted into an insoluble blue-colored...

Dietary zinc restriction and repletion affects DNA integrity in healthy men
Yang Song, Carolyn S Chung, Richard S Bruno, Maret G Traber, Kenneth H Brown, Janet C King, and Emily Ho
Am. J. Clinical Nutrition, Aug 2009; 90: 321 - 328.
...mixed with 200 muL 0.5% low-melting-point agarose and applied onto microscope slides (Trevigen, Gaithersburg, MD). Slides were stored in lysis buffer (Trevigen, Gaithersburg, MD) and shipped to Oregon State University within 3 d. At Oregon State...

1-Adrenergic Receptors Regulate Neurogenesis and Gliogenesis
Manveen K. Gupta, Robert S. Papay, Chris W. D. Jurgens, Robert J. Gaivin, Ting Shi, Van A. Doze, and Dianne M. Perez
Mol. Pharmacol., Aug 2009; 76: 314 - 326.
...detection. Apoptosis Assay. Neurospheres were grown and analyzed for apoptosis by using the FlowTACS Apoptosis Detection Kit (Trevigen, Gaithersburg, MD). Terminal deoxynucleotidyl transferase end-labeling of the free 3-hydroxyl residues in the fragmented DNA...

PP2A-dependent Dephosphorylation of Replication Protein A (RPA) is Required for the Repair of DNA breaks Induced by Replication Stress
Junjie Feng, Timothy Wakeman, Sheila Yong, Xiaohua Wu, Sally Kornbluth, and Xiao-Fan Wang
Mol. Cell. Biol., Aug 2009; 10.1128/MCB.00191-09.
...repair kinetics of HU-induced DNA breaks was evaluated by the alkaline comet2 assay according to the manufacturer's protocol (Trevigen). Briefly, cells were pulse-3 exposed to HU (0.2 mM, 24 hr) and harvested at various recovery time points for single-4 cell...

CD133+ Glioblastoma Stem-like Cells are Radiosensitive with a Defective DNA Damage Response Compared with Established Cell Lines
Amy M. McCord, Muhammad Jamal, Eli S. Williams, Kevin Camphausen, and Philip J. Tofilon
Clin. Cancer Res., Aug 2009; 15: 5145 - 5153.
...the neutral comet assay was done using a commercially available kit according to the recommendations from the manufacturer (Trevigen) with slight modifications. Briefly, monolayer cultures were irradiated (10 Gy), and at specified times, single-cell suspensions...

Nucleophosmin Redistribution following Heat Shock: A Role in Heat-Induced Radiosensitization
Robert P. Vanderwaal, Leonard B. Maggi, Jr., Jason D. Weber, Clayton R. Hunt, and Joseph L. Roti Roti
Cancer Res., Aug 2009; 69: 6454 - 6462.
...on an Olympus fluorescent microscope. Assay for DSBs. The Neutral Comet Assay was performed using the Comet Assay kit from Trevigen following manufacturers instructions. Proteinase K (Sigma), when used, was added to the lysis solution (0.27 U/mL). Lysis occurred...

A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases
Dorjbal Dorjsuren, David M. Wilson, III, William A. Beard, John P. McDonald, Christopher P. Austin, Roger Woodgate, Samuel H. Wilson, and Anton Simeonov
Nucleic Acids Res., Aug 2009; 10.1093/nar/gkp641.
...plates following previously described protocols (27). Enzymes Recombinant human DNA pol beta was either purchased from Trevigen Inc. (Gaithersburg, MD) or produced via published protocols (28). Equivalent performance was noted for the enzyme obtained...

Modulation of Carcinogen Metabolism by Nitric Oxide-Aspirin 2 Is Associated with Suppression of DNA Damage and DNA Adduct Formation
Christopher J. MacDonald, Robert Y. S. Cheng, David D. Roberts, David A. Wink, and Grace Chao Yeh
J. Biol. Chem., Aug 2009; 284: 22099 - 22107.
...Single cell gel electrophoresis assay (31) with alkaline electrophoresis was performed according to the CometAssay protocol (Trevigen, Gaithersburg, MD), and under protective yellow lighting. In brief, HepG2 cells were treated with either 10 mum BP or 10 nm...

Synergistic effects of autologous cell and hepatocyte growth factor gene therapy for neovascularization in the murine model of hindlimb ischemia.
Yasutaka Yamamoto, Takashi Matsuura, Genta Narazaki, Miyoko Sugitani, Kohei Tanaka, Akihiro Maeda, Goshi Shiota, Kenzo Sato, Akio Yoshida, and Ichiro Hisatome
Am J Physiol Heart Circ Physiol, Aug 2009; 10.1152/ajpheart.00321.2009.
...terminal deoxynucleotide168 transferase-mediated dUTP nick end labeling (TUNEL) using a commercially169 available kit (TACS/XL, Trevigen, Gaithersburg, MD).170 171 Migration assay172 12 A total of 4 ? 105 aMNC in 0.5 mL of DMEM with 0.4% FBS were seeded173 in...

The cytotoxic T lymphocyte protease granzyme A cleaves and inactivates poly(adenosine 5'-diphosphate-ribose) polymerase-1
Pengcheng Zhu, Denis Martinvalet, Dipanjan Chowdhury, Dong Zhang, Ann Schlesinger, and Judy Lieberman
Blood, Aug 2009; 114: 1205 - 1216.
...and goat polyclonal antibodies to C23 (F-18), N-terminal and C-terminal regions of PARP-1, and PARP-2 (Santa Cruz), and PAR (Trevigen, Inc). HRP-conjugated donkey anti-goat IgG (Santa Cruz), HRP-conjugated donkey anti-rabbit and anti-mouse IgG (Amersham) were...

Requirement for Protein Synthesis at Developing Synapses
Joseph Sebeo, Kuangfu Hsiao, Ozlem Bozdagi, Dani Dumitriu, Yongchao Ge, Qiang Zhou, and Deanna L. Benson
J. Neurosci., Aug 2009; 29: 9778 - 9793.
...Sinai School of Medicine, New York, NY), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (rabbit polyclonal; 1:1000; Trevigen). Fluorescence-tagged secondary antibodies were obtained from Vector Laboratories and Jackson ImmunoResearch. For WB, species-appropriate...

Prolonged Cell Cycle Response of HeLa Cells to Low-Level Alkylation Exposure
Allen G. Schroering, Anbarasi Kothandapani, Steve M. Patrick, Saravanan Kaliyaperumal, Vishal P. Sharma, and Kandace J. Williams
Cancer Res., Aug 2009; 69: 6307 - 6314.
...The slides were then placed into neutralizing solution [0.4 mol/L Tris-HCl (pH 7.5)] for 10 min and stained with SYBR Green (Trevigen). Comets were scored using a Nikon epifluorescence microscope at 200 magnification. Fifty cells were analyzed per slide using...

SIRT1 Promotes Cell Survival under Stress by Deacetylation-Dependent Deactivation of Poly(ADP-Ribose) Polymerase 1
Senthilkumar B. Rajamohan, Vinodkumar B. Pillai, Madhu Gupta, Nagalingam R. Sundaresan, Konstantin G. Birukov, Sadhana Samant, Michael O. Hottiger, and Mahesh P. Gupta
Mol. Cell. Biol., Aug 2009; 29: 4116 - 4129.
...autoradiography or Western blotting with the appropriate antibodies. In experiments where the effect of DNA was examined, activated DNA (Trevigen) or genomic DNA (1 ug/sample) was added to the buffer before incubating PARP1 with radiolabeled NAD. Cell death assay...