Trevigen releases human placenta derived basement membrane extract providing a pathogen and xenographic free microenvironment for human cell research. Basement Membrane Extract (BME) can be used for promotion and maintenance of a specific phenotype in a variety of cell cultures including stem cells, primary epithelial cells, primary endothelial cells and smooth muscle cells.
A basement membrane extract derived from mouse EHS sarcoma is the historical standard with which research has been done on a variety of normal and cancer cells derived from both animal and human sources. While these models do demonstrate a physiological response, there are subtle differences in the composition of the extracellular matrix as a result of tissue source and species-specificity, as it is known that there are alterations in the tumor microenvironment, and mouse-specific proteins may impact the normal human cellular response.
As an option to avoid these issues, Trevigen has developed a BME derived from normal human placenta, avoiding the presence of both tumor-derived and mouse-specific proteins in the human cell microenvironment. The human BME has been functionally tested for compatibility with human cells. The human BME has also been tested for sterility, and further PCR tested to insure the absence of eight human pathogenic viruses including Hepatitis A, B and C, HIV 1 and 2, Hantaan, Seoul and Sin Nombre.
Trevigen, Inc. is a rapidly growing biotechnology company focused on the development of products and technology for cancer research, emphasizing apoptosis, DNA damage and repair, and cancer cell function and behavior. Trevigen has been a long-standing provider of quality reagents and kits for researchers investigating programmed cell death and DNA damage and repair. A logical extension of the focus on cancer research has been the recent development of assays for cancer cell function and behavior including angiogenesis, cell invasion and tumor formation. Currently, the product portfolio contains over 500 products categorized into four processes – Apoptosis, DNA Damage and Repair, Cancer Cell Behavior, and Oxidative Stress.
Trevigen, Inc. provides a complete line of specialized proteins for cell research, cancer cell assays and recently Mesenchymal stem cell differentiation products. Visit www.trevigen.com or contact us at 1-800-873-8443 or info@trevigen.com for more information.
Thursday, August 20, 2009
Friday, August 7, 2009
Recently published scientific papers citing the use of Trevigen products
Laminin-1 induces E-cadherin expression in 3-dimensional cultured breast cancer cells by inhibiting DNA methyltransferase 1 and reversing promoter methylation statusGabriel Benton, Elliott Crooke, and Jay GeorgeFASEB J, Jul 2009; 10.1096/fj.08-128702.
...dehydrogenase (GAPDH) was obtained from Trevigen (Gaithersburg, MD, USA). Secondary antibodies...
GAPDH depletion induces cell cycle arrest and resistance to antimetabolites in human carcinoma cell linesManali S Phadke, Natalia F Krynetskaia, Anurag K Mishra, and Evgeny KrynetskiyJ. Pharmacol. Exp. Ther., Jul 2009; 10.1124/jpet.109.155671.
...were detected by Neutral Comet assay using the CometAssay kit (Trevigen, Gaithersburg, MD), according to manufacturer's instructions...
...with LMA agarose (1:10 v/v), and applied on the FLARE slides (Trevigen, Gaithersburg, MD). After cell lysis in a neutral lysis buffer...
Nicked 2-glycoprotein I binds angiostatin4.5 (plasminogen kringle 1-5) and attenuates its anti-angiogenic propertyHisako Nakagawa, Shinsuke Yasuda, Eiji Matsuura, Kazuko Kobayashi, Masahiro Ieko, Hiroshi Kataoka, Tetsuya Horita, Tatsuya Atsumi, and Takao KoikeBlood, Jul 2009; 10.1182/blood-2008-12-190629.
...angiogenesis assay (DIVAA). The DIVAA assay was obtained from Trevigen Inc. (Gaithersburg, MD) and performed as previously described...
Transient dsDNA breaks during pre-replication complex assemblyEmmanouil Rampakakis and Maria Zannis-HadjopoulosNucleic Acids Res., Jul 2009; 10.1093/nar/gkp617.
...Electrophoretic mobility-shift assay (EMSA) Nuclear cell extracts (10 mug) or 100 ng of recombinant Ku (Trevigen)...
Nucleophosmin Redistribution following Heat Shock: A Role in Heat-Induced RadiosensitizationRobert P. Vanderwaal, Leonard B. Maggi, Jr., Jason D. Weber, Clayton R. Hunt, and Joseph L. Roti RotiCancer Res., Jul 2009; 10.1158/0008-5472.CAN-08-4896.
...The Neutral Comet Assay was performed using the CometAssay kit from Trevigen following manufacturer's instructions...
Estrogen Inhibits ATR Signaling to Cell Cycle Checkpoints and DNA RepairAli Pedram, Mahnaz Razandi, Albert J. Evinger, Eva Lee, and Ellis R. LevinMol. Biol. Cell, Jul 2009; 20: 3374 - 3389.
...subjected to electrophoresis. The cells were stained with DNA intercalating dye (SYBR green and visualized by epifluorescence (Trevigen, Gaithersburg, MD; UVDE Flare Assay Kit). Damaged, unrepaired DNA was seen as the tail of the Comet, quantified from 200 cells...
Regulation of transgenes in three-dimensional cultures of primary mouse mammary cells demonstrates oncogene dependence and identifies cells that survive deinductionMartin Jechlinger, Katrina Podsypanina, and Harold VarmusGenes & Dev., Jul 2009; 23: 1677 - 1688.
...Materials 3D Culture Matrix Basement Membrane Extract (Cat# 3445-048-01) and Rat Collagen I (Cat#3447-020-01) were purchased from Trevigen Inc...
The Fowlpox Virus BCL-2 Homologue, FPV039, Interacts with Activated Bax and a Discrete Subset of BH3-Only Proteins To Inhibit ApoptosisLogan Banadyga, Kirstin Veugelers, Stephanie Campbell, and Michele BarryJ. Virol., Jul 2009; 83: 7085 - 7098.
...mouse anti-Bax2D2 (1:10,000; Trevigen), mouse anti-Flag HRP (1:2,000; Sigma-Aldrich), mouse anti-Myc (clone 9E10; 1:2,500), mouse anti-T7 Tag...
Chelation of intracellular iron with the anti-fungal agent ciclopirox olamine induces cell death in leukemia and myeloma cellsYanina Eberhard, Sean McDermott, Xiaoming Wang, Marcela Gronda, Amudha Venugopal, Tabitha E. Wood, Rose Hurren, Alessandro Datti, Robert A. Batey, Jeffrey Wrana, William E. Antholine, John Dick, and Aaron D. SchimmerBlood, Jul 2009; 10.1182/blood-2009-03-209965.
...or with mouse antihuman GAPDH (Trevigen, Gaithersburg, MD). Secondary antibodies were horseradish peroxidase-conjugated...
The immune adaptor molecule SARM modulates TNF- production and microglia activation in the brainstem and restricts West Nile virus pathogenesisKristy J. Szretter, Melanie A. Samuel, Susan Gilfillan, Anja Fuchs, Marco Colonna, and Michael S. DiamondJ. Virol., Jul 2009; 10.1128/JVI.00836-09.
...TUNEL staining was performed using the NeuroTACS II In Situ Apoptosis Detection kit (Trevigen) according to the manufacturer's protocols with some modifications...
DNA Damage-Induced Phosphorylation of TRF2 Is Required for the Fast Pathway of DNA Double-Strand Break RepairNazmul Huda, Hiromi Tanaka, Marc S. Mendonca, and David GilleyMol. Cell. Biol., Jul 2009; 29: 3597 - 3604.
...To study DNA DSB repair, neutral comet assays were performed using CometAssaykits (Trevigen). Induced and uninduced cells were prepared and exposed to X rays at 20 Gy on ice...
Zip3 (Slc39a3) functions in zinc reuptake from the alveolar lumen in lactating mammary glandShannon L. Kelleher, Veronica Lopez, Bo Lönnerdal, Jodi Dufner-Beattie, and Glen K. AndrewsAm J Physiol Regulatory Integrative Comp Physiol, Jul 2009; 297: R194 - R201.
...apoptotic cells in the mammary gland was assessed in situ using the TACS-XL Basic In Situ Apoptosis (TUNEL) Detection kit (Trevigen, Gaithersburg, MD) per the manufacturers instructions...
...dehydrogenase (GAPDH) was obtained from Trevigen (Gaithersburg, MD, USA). Secondary antibodies...
GAPDH depletion induces cell cycle arrest and resistance to antimetabolites in human carcinoma cell linesManali S Phadke, Natalia F Krynetskaia, Anurag K Mishra, and Evgeny KrynetskiyJ. Pharmacol. Exp. Ther., Jul 2009; 10.1124/jpet.109.155671.
...were detected by Neutral Comet assay using the CometAssay kit (Trevigen, Gaithersburg, MD), according to manufacturer's instructions...
...with LMA agarose (1:10 v/v), and applied on the FLARE slides (Trevigen, Gaithersburg, MD). After cell lysis in a neutral lysis buffer...
Nicked 2-glycoprotein I binds angiostatin4.5 (plasminogen kringle 1-5) and attenuates its anti-angiogenic propertyHisako Nakagawa, Shinsuke Yasuda, Eiji Matsuura, Kazuko Kobayashi, Masahiro Ieko, Hiroshi Kataoka, Tetsuya Horita, Tatsuya Atsumi, and Takao KoikeBlood, Jul 2009; 10.1182/blood-2008-12-190629.
...angiogenesis assay (DIVAA). The DIVAA assay was obtained from Trevigen Inc. (Gaithersburg, MD) and performed as previously described...
Transient dsDNA breaks during pre-replication complex assemblyEmmanouil Rampakakis and Maria Zannis-HadjopoulosNucleic Acids Res., Jul 2009; 10.1093/nar/gkp617.
...Electrophoretic mobility-shift assay (EMSA) Nuclear cell extracts (10 mug) or 100 ng of recombinant Ku (Trevigen)...
Nucleophosmin Redistribution following Heat Shock: A Role in Heat-Induced RadiosensitizationRobert P. Vanderwaal, Leonard B. Maggi, Jr., Jason D. Weber, Clayton R. Hunt, and Joseph L. Roti RotiCancer Res., Jul 2009; 10.1158/0008-5472.CAN-08-4896.
...The Neutral Comet Assay was performed using the CometAssay kit from Trevigen following manufacturer's instructions...
Estrogen Inhibits ATR Signaling to Cell Cycle Checkpoints and DNA RepairAli Pedram, Mahnaz Razandi, Albert J. Evinger, Eva Lee, and Ellis R. LevinMol. Biol. Cell, Jul 2009; 20: 3374 - 3389.
...subjected to electrophoresis. The cells were stained with DNA intercalating dye (SYBR green and visualized by epifluorescence (Trevigen, Gaithersburg, MD; UVDE Flare Assay Kit). Damaged, unrepaired DNA was seen as the tail of the Comet, quantified from 200 cells...
Regulation of transgenes in three-dimensional cultures of primary mouse mammary cells demonstrates oncogene dependence and identifies cells that survive deinductionMartin Jechlinger, Katrina Podsypanina, and Harold VarmusGenes & Dev., Jul 2009; 23: 1677 - 1688.
...Materials 3D Culture Matrix Basement Membrane Extract (Cat# 3445-048-01) and Rat Collagen I (Cat#3447-020-01) were purchased from Trevigen Inc...
The Fowlpox Virus BCL-2 Homologue, FPV039, Interacts with Activated Bax and a Discrete Subset of BH3-Only Proteins To Inhibit ApoptosisLogan Banadyga, Kirstin Veugelers, Stephanie Campbell, and Michele BarryJ. Virol., Jul 2009; 83: 7085 - 7098.
...mouse anti-Bax2D2 (1:10,000; Trevigen), mouse anti-Flag HRP (1:2,000; Sigma-Aldrich), mouse anti-Myc (clone 9E10; 1:2,500), mouse anti-T7 Tag...
Chelation of intracellular iron with the anti-fungal agent ciclopirox olamine induces cell death in leukemia and myeloma cellsYanina Eberhard, Sean McDermott, Xiaoming Wang, Marcela Gronda, Amudha Venugopal, Tabitha E. Wood, Rose Hurren, Alessandro Datti, Robert A. Batey, Jeffrey Wrana, William E. Antholine, John Dick, and Aaron D. SchimmerBlood, Jul 2009; 10.1182/blood-2009-03-209965.
...or with mouse antihuman GAPDH (Trevigen, Gaithersburg, MD). Secondary antibodies were horseradish peroxidase-conjugated...
The immune adaptor molecule SARM modulates TNF- production and microglia activation in the brainstem and restricts West Nile virus pathogenesisKristy J. Szretter, Melanie A. Samuel, Susan Gilfillan, Anja Fuchs, Marco Colonna, and Michael S. DiamondJ. Virol., Jul 2009; 10.1128/JVI.00836-09.
...TUNEL staining was performed using the NeuroTACS II In Situ Apoptosis Detection kit (Trevigen) according to the manufacturer's protocols with some modifications...
DNA Damage-Induced Phosphorylation of TRF2 Is Required for the Fast Pathway of DNA Double-Strand Break RepairNazmul Huda, Hiromi Tanaka, Marc S. Mendonca, and David GilleyMol. Cell. Biol., Jul 2009; 29: 3597 - 3604.
...To study DNA DSB repair, neutral comet assays were performed using CometAssaykits (Trevigen). Induced and uninduced cells were prepared and exposed to X rays at 20 Gy on ice...
Zip3 (Slc39a3) functions in zinc reuptake from the alveolar lumen in lactating mammary glandShannon L. Kelleher, Veronica Lopez, Bo Lönnerdal, Jodi Dufner-Beattie, and Glen K. AndrewsAm J Physiol Regulatory Integrative Comp Physiol, Jul 2009; 297: R194 - R201.
...apoptotic cells in the mammary gland was assessed in situ using the TACS-XL Basic In Situ Apoptosis (TUNEL) Detection kit (Trevigen, Gaithersburg, MD) per the manufacturers instructions...
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