Senescence-Associated Oxidative DNA Damage Promotes the Generation of Neoplastic Cells
Karo Gosselin, Sébastien Martien, Albin Pourtier, Chantal Vercamer, Peter Ostoich, Luc Morat, Laure Sabatier, Laurence Duprez, Claire T'Kint de Roodenbeke, Eric Gilson, Nicolas Malaquin, Nicolas Wernert, Predrag Slijepcevic, Marjan Ashtari, Fazia Chelli, Emeric Deruy, Bernard Vandenbunder, Yvan De Launoit, and Corinne Abbadie
Cancer Res., Oct 2009; 69: 7917 – 7925.
...suspension was immediately laid onto a Trevigen cometslide. Agarose was allowed to solidify...and stained with either SYBR Green I (Trevigen) according to the manufacturers recommendations...oxo-7,8-dihydroguanine (8-oxoG) antibody is from Trevigen. Because it recognizes 8-oxoG in both...
Glyceraldehyde 3-Phosphate Dehydrogenase Depletion Induces Cell Cycle Arrest and Resistance to Antimetabolites in Human Carcinoma Cell Lines
Manali S. Phadke, Natalia F. Krynetskaia, Anurag K. Mishra, and Evgeny Krynetskiy
J. Pharmacol. Exp. Ther., Oct 2009; 331: 77 – 86.
...were detected by Neutral Comet assay with the CometAssay kit (Trevigen, Gaithersburg, MD), according to the manufacturer's instructions...Fragment Length Analysis using Repair Enzymes (FLARE) slides (Trevigen). After cell lysis in a neutral lysis buffer at 4C overnight...
Anti-angiogenic activity of carebastine: a plausible mechanism affecting airway remodelling
A. De Luisi, G. Mangialardi, R. Ria, G. Acuto, D. Ribatti, and A. Vacca
Eur. Respir. J., Oct 2009; 34: 958 – 966.
...24-well plates pre-coated with Cultrex basement membrane extract Trevigen, Inc., Gaithersburg, MD, USA; 400uLwell in 1mLwell of SFM...endothelial cells were seeded on Cultrex basement membrane extract Trevigen, Inc., Gaithersburg, MD, USA in: a serum-free medium SFM alone...
Udu Deficiency Activates DNA Damage Checkpoint
Chiaw-Hwee Lim, Shang-Wei Chong, and Yun-Jin Jiang
Mol. Biol. Cell, Oct 2009; 20: 4183 – 4193.
...assay was performed using the CometAssay kit (4250-040-K; Trevigen, Gaithersburg, MD) according to the manufacturer's instruction. DNA was stained with SYBR Green as provided (Trevigen). Comet images were taken by fluorescence microscopy...
Hepatitis B and Hepatitis C virus replication up-regulate serine protease inhibitor Kazal, resulting in cellular resistance to serine protease dependent apoptosis
Jason Lamontagne, Mark Pinkerton, Timothy M. Block, and Xuanyong Lu
J. Virol., Oct 2009; 10.1128/JVI.01249-09.
...BFA/CHX/Z-VAD122 7 treated cells were washed with PBS and then incubated with annexin V-FITC in 100ul of binding123 buffer (Trevigen Inc., Gaithersburg, MD). After incubating for 30 minutes, the cells were washed124 with PBS and were then visualized with a...
Proteasome-dependent Processing of Topoisomerase I-DNA Adducts into DNA Double Strand Breaks at Arrested Replication Forks
Chao-Po Lin, Yi Ban, Yi Lisa Lyu, and Leroy F. Liu
J. Biol. Chem., Oct 2009; 284: 28084 – 28092.
...streptomycin (100 mug/ml) in a 30 C incubator with 5% CO2. Neutral Comet Assay The comet assays were performed according to the Trevigen CometAssayTM kit protocol with slight modifications. Cells were pretreated with various inhibitors for 30 min, followed by...
In Utero Exposure to Di-(2-Ethylhexyl) Phthalate Decreases Mineralocorticoid Receptor Expression in the Adult Testis
D. B. Martinez-Arguelles, M. Culty, B. R. Zirkin, and V. Papadopoulos
Endocrinology, Oct 2009; 10.1210/en.2009-0847.
...difluoride membrane. The membrane was incubated with antiserum against MR (see above) and then against GAPDH (2275-PC-100; Trevigen, Gaithersburg, MD). Proteins were detected with a secondary antibody coupled to horseradish peroxidase (goat antimouse, 554002...
Effects of Sp1 overexpression on cultured human corneal stromal cells
Xiang Shen, Jeong-Seok Park, Ye Qiu, Joel Sugar, and Beatrice Y J T Yue
Genes Cells, Oct 2009; 14: 1133 - 1139.
...anti-cathepsin B, anti-a1-PI or anti-glyceraldehyde 3-phosphate dehydrogenase (Trevigen, Gaithersburg, MD, USA). Horseradish peroxidase-conjugated goat anti-rabbit IgG was used as the secondary...
Poly(Adenosine 5'-Diphosphate-Ribose) Polymerase Inhibition Counteracts Multiple Manifestations of Experimental Type 1 Diabetic Nephropathy
Viktor R. Drel, Weizheng Xu, Jie Zhang, Ivan A. Pavlov, Hanna Shevalye, Barbara Slusher, and Irina G. Obrosova
Endocrinology, Oct 2009; 10.1210/en.2009-0628.
...Mouse monoclonal anti-poly(ADP-ribose) antibody was purchased from Trevigen, Inc. (Gaithersburg, MD), rabbit poly-clonal antinitrotyrosine (NT) antibody and mouse monoclonal anti-NT antibody...
Development and Characterization of SSR Markers and Their Use to Assess Genetic Relationships among Alfalfa Germplasms
C. He, Z. L. Xia, T. A. Campbell, and G. R. Bauchan
Crop Sci., Oct 2009; 49: 2176 – 2186.
...PCR products were finally extended at 72C for 5 min. DNA fragments were sized using high-resolution 3% TREVIGEL agarose gels (Trevigen, Inc., Gaithersburg, MD) or the ABI PRISM 3100 Genetic Analyzer. Nomenclature for SSR Markers SSR names were prefixed with...
TSLP Induces Chemotactic and Pro-survival Effects in Eosinophils: Implications in Allergic Inflammation
Chun K Wong, Shuiqing Hu, Phyllis F.Y. Cheung, and Christopher WK Lam
Am. J. Respir. Cell Mol. Biol., Oct 2009; 10.1165/rcmb.2009-0168OC.
...Walkersville, MD). Page 8 of 56 9 Apoptosis assay Apoptosis of eosinophils was assessed by the TACS Annexin V-FITC assay (Trevigen, Gaithersburg, MD, USA) using flow cytometry. The population of viable...
Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells
Sajiv Chandradas, Gintaras Deikus, Jonathan G. Tardos, and Vladimir Y. Bogdanov
J. Leukoc. Biol., Oct 2009; 10.1189/jlb.0409252.
...lysates, anti-SR protein mAb 16H3. The blot was reprobed with anti-GAPDH polyclonal antibody (Trevigen, Gaithersburg, MD, USA). (B) In silico analysis of TF exon 5 using web resources ESEfinder...
Oral Poly(ADP-Ribose) Polymerase-1 Inhibitor BSI-401 Has Antitumor Activity and Synergizes with Oxaliplatin against Pancreatic Cancer, Preventing Acute Neurotoxicity
Davide Melisi, Valeria Ossovskaya, Cihui Zhu, Roberta Rosa, Jianhua Ling, Patrick M. Dougherty, Barry M. Sherman, James L. Abbruzzese, and Paul J. Chiao
Clin. Cancer Res., Oct 2009; 15: 6367 – 6377.
...activity of PARP in human pancreatic cancer cell lines and MEFs was measured using a universal colorimetric PARP assay kit (Trevigen). Apoptosis The extent of apoptosis was determined by fragmented DNA detection as previously described by Matassov...
Hepatitis C Viral Proteins induce NADPH Oxidase 4 (Nox4) in a TGF-dependent manner: A new contributor of HCV-induced oxidative stress
Howard E. Boudreau, Suzanne U. Emerson, Agnieszka Korzeniowska, Meghan A. Jendrysik, and Thomas L. Leto
J. Virol., Oct 2009; 10.1128/JVI.01059-09.
...antibody, rabbit polyclonal anti-Actin antibody, rabbit polyclonal anti-GAPDH (Trevigen, Gaithersburg, MD, USA), mouse monoclonal anti-V5, goat polyclonal anti-E2...
Acquired Resistance to Combination Treatment with Temozolomide and ABT-888 Is Mediated by Both Base Excision Repair and Homologous Recombination DNA Repair Pathways
Xuesong Liu, Edward K. Han, Mark Anderson, Yan Shi, Dimitri Semizarov, Gang Wang, Thomas McGonigal, Lisa Roberts, Loren Lasko, Joann Palma, Gui-dong Zhu, Thomas Penning, Saul Rosenberg, Vincent L. Giranda, Yan Luo, Joel Leverson, Eric F. Johnson, and Alexander R. Shoemaker
Mol. Cancer Res., Oct 2009; 7: 1686 – 1692.
...Polyclonal anti-PAR antibody was from Trevigen. Western blot analysis was done using enhanced chemiluminescence detection reagents according to the instructions...
Synergistic effects of autologous cell and hepatocyte growth factor gene therapy for neovascularization in a murine model of hindlimb ischemia
Yasutaka Yamamoto, Takashi Matsuura, Genta Narazaki, Miyoko Sugitani, Kohei Tanaka, Akihiro Maeda, Goshi Shiota, Kenzo Sato, Akio Yoshida, and Ichiro Hisatome
Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1329 - H1336.
...by terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) using a commercially available kit (TACS/XL, Trevigen, Gaithersburg, MD). Migration assay. A total of 4 105 aMNCs in 0.5 ml DMEM with 0.4% FBS were seeded in the upper chamber...
Translocator Protein Blockade Reduces Prostate Tumor Growth
Arlee Fafalios, Ardavan Akhavan, Anil V. Parwani, Robert R. Bies, Kevin J. McHugh, and Beth R. Pflug
Clin. Cancer Res., Oct 2009; 15: 6177 – 6184.
...Slides were then loaded on a Dako Autostainer. The primary anti-TSPO (working dilution, 1:350) was a polyclonal rabbit antibody (Trevigen). The immunolabeling procedures were done using Dako EnVision-Labeled Polymer-HRP antirabbit and were carried out according...
Vorinostat Inhibits Brain Metastatic Colonization in a Model of Triple-Negative Breast Cancer and Induces DNA Double-Strand Breaks
Diane Palmieri, Paul R. Lockman, Fancy C. Thomas, Emily Hua, Jeanne Herring, Elizabeth Hargrave, Matthew Johnson, Natasha Flores, Yongzhen Qian, Eleazar Vega-Valle, Kunal S. Taskar, Vinay Rudraraju, Rajendar K. Mittapalli, Julie A. Gaasch, Kaci A. Bohn, Helen R. Thorsheim, David J. Liewehr, Sean Davis, John F. Reilly, Robert Walker, Julie L. Bronder, Lionel Feigenbaum, Seth M. Steinberg, Kevin Camphausen, Paul S. Meltzer, Victoria M. Richon, Quentin R. Smith, and Patricia S. Steeg
Clin. Cancer Res., Oct 2009; 15: 6148 – 6157.
...migration assay Boyden chamber motility assays were conducted as described previously (8). Neutral comet assay kit from Trevigen was used according to the recommendations from the manufacturer. DNA was stained with propidium iodide and digital fluorescent...
A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases
Dorjbal Dorjsuren, David M. Wilson, III, William A. Beard, John P. McDonald, Christopher P. Austin, Roger Woodgate, Samuel H. Wilson, and Anton Simeonov
Nucleic Acids Res., Oct 2009; 37: e128.
...plates following previously described protocols (27). Enzymes Recombinant human DNA pol beta was either purchased from Trevigen (Gaithersburg, MD) or produced via published protocols (28). Equivalent performance was noted for the enzyme obtained...
Chelation of intracellular iron with the antifungal agent ciclopirox olamine induces cell death in leukemia and myeloma cells
Yanina Eberhard, Sean P. McDermott, Xiaoming Wang, Marcela Gronda, Amudha Venugopal, Tabitha E. Wood, Rose Hurren, Alessandro Datti, Robert A. Batey, Jeffrey Wrana, William E. Antholine, John Dick, and Aaron D. Schimmer
Blood, Oct 2009; 114: 3064 - 3073.
...Membranes were probed with polyclonal rabbit anti-human survivin (1 mug/mL), or with mouse anti-human GAPDH (Trevigen). Secondary antibodies were horseradish peroxidase-conjugated goat anti-mouse IgG (1:10 000 [vol/vol]) and...
Monday, November 2, 2009
Monday, September 21, 2009
New Citations using Trevigen Products
The Recombination Protein RAD52 Cooperates with the Excision Repair Protein OGG1 for the Repair of Oxidative Lesions in Mammalian Cells
Nadja C. de Souza-Pinto, Scott Maynard, Kazunari Hashiguchi, Jingping Hu, Meltem Muftuoglu, and Vilhelm A. Bohr
Mol. Cell. Biol., Aug 2009; 29: 4441 - 4454.
...incubated in primary anti-8-oxoG antibody (Trevigen) at a 1:250 dilution in 10 goat serum-PBS...heterochromatic DNA (7, 32, 40; see also the Trevigen web site). For the control, the results...8-oxoG antibody (Fig. 7C) that was used (Trevigen) in two ways. First, treatment of HeLa...
Poly(ADP-ribose) polymerase inhibitor ABT-888 potentiates the cytotoxic activity of temozolomide in leukemia cells: influence of mismatch repair status and O6-methylguanine-DNA methyltransferase activity
Terzah M. Horton, Gaye Jenkins, Debananda Pati, Linna Zhang, M. Eileen Dolan, Albert Ribes-Zamora, Alison A. Bertuch, Susan M. Blaney, Shannon L. Delaney, Madhuri Hegde, and Stacey L. Berg
Mol. Cancer Ther., Aug 2009; 8: 2232 - 2242.
...a commercially available PARP assay (Trevigen). Cell lysates were prepared from 1 107 leukemia cells using 1 PARP buffer (Trevigen) supplemented with 0.4 mmol/L phenylmethylsulfonyl...in triplicate, and recombinant PARP (Trevigen) was used to produce a standard curve...
Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler
Aaron J. Gottschalk, Gyula Timinszky, Stephanie E. Kong, Jingji Jin, Yong Cai, Selene K. Swanson, Michael P. Washburn, Laurence Florens, Andreas G. Ladurner, Joan W. Conaway, and Ronald C. Conaway
PNAS, Aug 2009; 106: 13770 - 13774.
...pmol) Flag-Alc1 (wild-type, E175Q, or D723A) from HEK293/FRT cells or SF9 cells, 115 ng (1 pmol) Parp1 (Trevigen), recombinant Parg (Trevigen), 34 muM nicotine adenine dinucleotide, and 150 ng mono- or oligonucleosomes from HeLa cells (37...
Differences in Immunolocalization of Kim-1, RPA-1, and RPA-2 in Kidneys of Gentamicin-, Cisplatin-, and Valproic Acid-Treated Rats: Potential Role of iNOS and Nitrotyrosine
Jun Zhang, Peter L. Goering, Parvaneh Espandiari, Martin Shaw, Joseph V. Bonventre, Vishal S. Vaidya, Ronald P. Brown, Joe Keenan, Cormac G. Kilty, Nakissa Sadrieh, and Joseph P. Hanig
Toxicol Pathol, Aug 2009; 37: 629 - 643.
...was detected using the TACS in situ apoptotic detection kit (Trevigen, Inc., Gaithersburg, MD). The TUNEL procedures were as follows...ten minutes. In the final step, uncolored soluble chromogen (Trevigen blue label) was enzymatically converted into an insoluble blue-colored...
Dietary zinc restriction and repletion affects DNA integrity in healthy men
Yang Song, Carolyn S Chung, Richard S Bruno, Maret G Traber, Kenneth H Brown, Janet C King, and Emily Ho
Am. J. Clinical Nutrition, Aug 2009; 90: 321 - 328.
...mixed with 200 muL 0.5% low-melting-point agarose and applied onto microscope slides (Trevigen, Gaithersburg, MD). Slides were stored in lysis buffer (Trevigen, Gaithersburg, MD) and shipped to Oregon State University within 3 d. At Oregon State...
1-Adrenergic Receptors Regulate Neurogenesis and Gliogenesis
Manveen K. Gupta, Robert S. Papay, Chris W. D. Jurgens, Robert J. Gaivin, Ting Shi, Van A. Doze, and Dianne M. Perez
Mol. Pharmacol., Aug 2009; 76: 314 - 326.
...detection. Apoptosis Assay. Neurospheres were grown and analyzed for apoptosis by using the FlowTACS Apoptosis Detection Kit (Trevigen, Gaithersburg, MD). Terminal deoxynucleotidyl transferase end-labeling of the free 3-hydroxyl residues in the fragmented DNA...
PP2A-dependent Dephosphorylation of Replication Protein A (RPA) is Required for the Repair of DNA breaks Induced by Replication Stress
Junjie Feng, Timothy Wakeman, Sheila Yong, Xiaohua Wu, Sally Kornbluth, and Xiao-Fan Wang
Mol. Cell. Biol., Aug 2009; 10.1128/MCB.00191-09.
...repair kinetics of HU-induced DNA breaks was evaluated by the alkaline comet2 assay according to the manufacturer's protocol (Trevigen). Briefly, cells were pulse-3 exposed to HU (0.2 mM, 24 hr) and harvested at various recovery time points for single-4 cell...
CD133+ Glioblastoma Stem-like Cells are Radiosensitive with a Defective DNA Damage Response Compared with Established Cell Lines
Amy M. McCord, Muhammad Jamal, Eli S. Williams, Kevin Camphausen, and Philip J. Tofilon
Clin. Cancer Res., Aug 2009; 15: 5145 - 5153.
...the neutral comet assay was done using a commercially available kit according to the recommendations from the manufacturer (Trevigen) with slight modifications. Briefly, monolayer cultures were irradiated (10 Gy), and at specified times, single-cell suspensions...
Nucleophosmin Redistribution following Heat Shock: A Role in Heat-Induced Radiosensitization
Robert P. Vanderwaal, Leonard B. Maggi, Jr., Jason D. Weber, Clayton R. Hunt, and Joseph L. Roti Roti
Cancer Res., Aug 2009; 69: 6454 - 6462.
...on an Olympus fluorescent microscope. Assay for DSBs. The Neutral Comet Assay was performed using the Comet Assay kit from Trevigen following manufacturers instructions. Proteinase K (Sigma), when used, was added to the lysis solution (0.27 U/mL). Lysis occurred...
A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases
Dorjbal Dorjsuren, David M. Wilson, III, William A. Beard, John P. McDonald, Christopher P. Austin, Roger Woodgate, Samuel H. Wilson, and Anton Simeonov
Nucleic Acids Res., Aug 2009; 10.1093/nar/gkp641.
...plates following previously described protocols (27). Enzymes Recombinant human DNA pol beta was either purchased from Trevigen Inc. (Gaithersburg, MD) or produced via published protocols (28). Equivalent performance was noted for the enzyme obtained...
Modulation of Carcinogen Metabolism by Nitric Oxide-Aspirin 2 Is Associated with Suppression of DNA Damage and DNA Adduct Formation
Christopher J. MacDonald, Robert Y. S. Cheng, David D. Roberts, David A. Wink, and Grace Chao Yeh
J. Biol. Chem., Aug 2009; 284: 22099 - 22107.
...Single cell gel electrophoresis assay (31) with alkaline electrophoresis was performed according to the CometAssay protocol (Trevigen, Gaithersburg, MD), and under protective yellow lighting. In brief, HepG2 cells were treated with either 10 mum BP or 10 nm...
Synergistic effects of autologous cell and hepatocyte growth factor gene therapy for neovascularization in the murine model of hindlimb ischemia.
Yasutaka Yamamoto, Takashi Matsuura, Genta Narazaki, Miyoko Sugitani, Kohei Tanaka, Akihiro Maeda, Goshi Shiota, Kenzo Sato, Akio Yoshida, and Ichiro Hisatome
Am J Physiol Heart Circ Physiol, Aug 2009; 10.1152/ajpheart.00321.2009.
...terminal deoxynucleotide168 transferase-mediated dUTP nick end labeling (TUNEL) using a commercially169 available kit (TACS/XL, Trevigen, Gaithersburg, MD).170 171 Migration assay172 12 A total of 4 ? 105 aMNC in 0.5 mL of DMEM with 0.4% FBS were seeded173 in...
The cytotoxic T lymphocyte protease granzyme A cleaves and inactivates poly(adenosine 5'-diphosphate-ribose) polymerase-1
Pengcheng Zhu, Denis Martinvalet, Dipanjan Chowdhury, Dong Zhang, Ann Schlesinger, and Judy Lieberman
Blood, Aug 2009; 114: 1205 - 1216.
...and goat polyclonal antibodies to C23 (F-18), N-terminal and C-terminal regions of PARP-1, and PARP-2 (Santa Cruz), and PAR (Trevigen, Inc). HRP-conjugated donkey anti-goat IgG (Santa Cruz), HRP-conjugated donkey anti-rabbit and anti-mouse IgG (Amersham) were...
Requirement for Protein Synthesis at Developing Synapses
Joseph Sebeo, Kuangfu Hsiao, Ozlem Bozdagi, Dani Dumitriu, Yongchao Ge, Qiang Zhou, and Deanna L. Benson
J. Neurosci., Aug 2009; 29: 9778 - 9793.
...Sinai School of Medicine, New York, NY), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (rabbit polyclonal; 1:1000; Trevigen). Fluorescence-tagged secondary antibodies were obtained from Vector Laboratories and Jackson ImmunoResearch. For WB, species-appropriate...
Prolonged Cell Cycle Response of HeLa Cells to Low-Level Alkylation Exposure
Allen G. Schroering, Anbarasi Kothandapani, Steve M. Patrick, Saravanan Kaliyaperumal, Vishal P. Sharma, and Kandace J. Williams
Cancer Res., Aug 2009; 69: 6307 - 6314.
...The slides were then placed into neutralizing solution [0.4 mol/L Tris-HCl (pH 7.5)] for 10 min and stained with SYBR Green (Trevigen). Comets were scored using a Nikon epifluorescence microscope at 200 magnification. Fifty cells were analyzed per slide using...
SIRT1 Promotes Cell Survival under Stress by Deacetylation-Dependent Deactivation of Poly(ADP-Ribose) Polymerase 1
Senthilkumar B. Rajamohan, Vinodkumar B. Pillai, Madhu Gupta, Nagalingam R. Sundaresan, Konstantin G. Birukov, Sadhana Samant, Michael O. Hottiger, and Mahesh P. Gupta
Mol. Cell. Biol., Aug 2009; 29: 4116 - 4129.
...autoradiography or Western blotting with the appropriate antibodies. In experiments where the effect of DNA was examined, activated DNA (Trevigen) or genomic DNA (1 ug/sample) was added to the buffer before incubating PARP1 with radiolabeled NAD. Cell death assay...
Nadja C. de Souza-Pinto, Scott Maynard, Kazunari Hashiguchi, Jingping Hu, Meltem Muftuoglu, and Vilhelm A. Bohr
Mol. Cell. Biol., Aug 2009; 29: 4441 - 4454.
...incubated in primary anti-8-oxoG antibody (Trevigen) at a 1:250 dilution in 10 goat serum-PBS...heterochromatic DNA (7, 32, 40; see also the Trevigen web site). For the control, the results...8-oxoG antibody (Fig. 7C) that was used (Trevigen) in two ways. First, treatment of HeLa...
Poly(ADP-ribose) polymerase inhibitor ABT-888 potentiates the cytotoxic activity of temozolomide in leukemia cells: influence of mismatch repair status and O6-methylguanine-DNA methyltransferase activity
Terzah M. Horton, Gaye Jenkins, Debananda Pati, Linna Zhang, M. Eileen Dolan, Albert Ribes-Zamora, Alison A. Bertuch, Susan M. Blaney, Shannon L. Delaney, Madhuri Hegde, and Stacey L. Berg
Mol. Cancer Ther., Aug 2009; 8: 2232 - 2242.
...a commercially available PARP assay (Trevigen). Cell lysates were prepared from 1 107 leukemia cells using 1 PARP buffer (Trevigen) supplemented with 0.4 mmol/L phenylmethylsulfonyl...in triplicate, and recombinant PARP (Trevigen) was used to produce a standard curve...
Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler
Aaron J. Gottschalk, Gyula Timinszky, Stephanie E. Kong, Jingji Jin, Yong Cai, Selene K. Swanson, Michael P. Washburn, Laurence Florens, Andreas G. Ladurner, Joan W. Conaway, and Ronald C. Conaway
PNAS, Aug 2009; 106: 13770 - 13774.
...pmol) Flag-Alc1 (wild-type, E175Q, or D723A) from HEK293/FRT cells or SF9 cells, 115 ng (1 pmol) Parp1 (Trevigen), recombinant Parg (Trevigen), 34 muM nicotine adenine dinucleotide, and 150 ng mono- or oligonucleosomes from HeLa cells (37...
Differences in Immunolocalization of Kim-1, RPA-1, and RPA-2 in Kidneys of Gentamicin-, Cisplatin-, and Valproic Acid-Treated Rats: Potential Role of iNOS and Nitrotyrosine
Jun Zhang, Peter L. Goering, Parvaneh Espandiari, Martin Shaw, Joseph V. Bonventre, Vishal S. Vaidya, Ronald P. Brown, Joe Keenan, Cormac G. Kilty, Nakissa Sadrieh, and Joseph P. Hanig
Toxicol Pathol, Aug 2009; 37: 629 - 643.
...was detected using the TACS in situ apoptotic detection kit (Trevigen, Inc., Gaithersburg, MD). The TUNEL procedures were as follows...ten minutes. In the final step, uncolored soluble chromogen (Trevigen blue label) was enzymatically converted into an insoluble blue-colored...
Dietary zinc restriction and repletion affects DNA integrity in healthy men
Yang Song, Carolyn S Chung, Richard S Bruno, Maret G Traber, Kenneth H Brown, Janet C King, and Emily Ho
Am. J. Clinical Nutrition, Aug 2009; 90: 321 - 328.
...mixed with 200 muL 0.5% low-melting-point agarose and applied onto microscope slides (Trevigen, Gaithersburg, MD). Slides were stored in lysis buffer (Trevigen, Gaithersburg, MD) and shipped to Oregon State University within 3 d. At Oregon State...
1-Adrenergic Receptors Regulate Neurogenesis and Gliogenesis
Manveen K. Gupta, Robert S. Papay, Chris W. D. Jurgens, Robert J. Gaivin, Ting Shi, Van A. Doze, and Dianne M. Perez
Mol. Pharmacol., Aug 2009; 76: 314 - 326.
...detection. Apoptosis Assay. Neurospheres were grown and analyzed for apoptosis by using the FlowTACS Apoptosis Detection Kit (Trevigen, Gaithersburg, MD). Terminal deoxynucleotidyl transferase end-labeling of the free 3-hydroxyl residues in the fragmented DNA...
PP2A-dependent Dephosphorylation of Replication Protein A (RPA) is Required for the Repair of DNA breaks Induced by Replication Stress
Junjie Feng, Timothy Wakeman, Sheila Yong, Xiaohua Wu, Sally Kornbluth, and Xiao-Fan Wang
Mol. Cell. Biol., Aug 2009; 10.1128/MCB.00191-09.
...repair kinetics of HU-induced DNA breaks was evaluated by the alkaline comet2 assay according to the manufacturer's protocol (Trevigen). Briefly, cells were pulse-3 exposed to HU (0.2 mM, 24 hr) and harvested at various recovery time points for single-4 cell...
CD133+ Glioblastoma Stem-like Cells are Radiosensitive with a Defective DNA Damage Response Compared with Established Cell Lines
Amy M. McCord, Muhammad Jamal, Eli S. Williams, Kevin Camphausen, and Philip J. Tofilon
Clin. Cancer Res., Aug 2009; 15: 5145 - 5153.
...the neutral comet assay was done using a commercially available kit according to the recommendations from the manufacturer (Trevigen) with slight modifications. Briefly, monolayer cultures were irradiated (10 Gy), and at specified times, single-cell suspensions...
Nucleophosmin Redistribution following Heat Shock: A Role in Heat-Induced Radiosensitization
Robert P. Vanderwaal, Leonard B. Maggi, Jr., Jason D. Weber, Clayton R. Hunt, and Joseph L. Roti Roti
Cancer Res., Aug 2009; 69: 6454 - 6462.
...on an Olympus fluorescent microscope. Assay for DSBs. The Neutral Comet Assay was performed using the Comet Assay kit from Trevigen following manufacturers instructions. Proteinase K (Sigma), when used, was added to the lysis solution (0.27 U/mL). Lysis occurred...
A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases
Dorjbal Dorjsuren, David M. Wilson, III, William A. Beard, John P. McDonald, Christopher P. Austin, Roger Woodgate, Samuel H. Wilson, and Anton Simeonov
Nucleic Acids Res., Aug 2009; 10.1093/nar/gkp641.
...plates following previously described protocols (27). Enzymes Recombinant human DNA pol beta was either purchased from Trevigen Inc. (Gaithersburg, MD) or produced via published protocols (28). Equivalent performance was noted for the enzyme obtained...
Modulation of Carcinogen Metabolism by Nitric Oxide-Aspirin 2 Is Associated with Suppression of DNA Damage and DNA Adduct Formation
Christopher J. MacDonald, Robert Y. S. Cheng, David D. Roberts, David A. Wink, and Grace Chao Yeh
J. Biol. Chem., Aug 2009; 284: 22099 - 22107.
...Single cell gel electrophoresis assay (31) with alkaline electrophoresis was performed according to the CometAssay protocol (Trevigen, Gaithersburg, MD), and under protective yellow lighting. In brief, HepG2 cells were treated with either 10 mum BP or 10 nm...
Synergistic effects of autologous cell and hepatocyte growth factor gene therapy for neovascularization in the murine model of hindlimb ischemia.
Yasutaka Yamamoto, Takashi Matsuura, Genta Narazaki, Miyoko Sugitani, Kohei Tanaka, Akihiro Maeda, Goshi Shiota, Kenzo Sato, Akio Yoshida, and Ichiro Hisatome
Am J Physiol Heart Circ Physiol, Aug 2009; 10.1152/ajpheart.00321.2009.
...terminal deoxynucleotide168 transferase-mediated dUTP nick end labeling (TUNEL) using a commercially169 available kit (TACS/XL, Trevigen, Gaithersburg, MD).170 171 Migration assay172 12 A total of 4 ? 105 aMNC in 0.5 mL of DMEM with 0.4% FBS were seeded173 in...
The cytotoxic T lymphocyte protease granzyme A cleaves and inactivates poly(adenosine 5'-diphosphate-ribose) polymerase-1
Pengcheng Zhu, Denis Martinvalet, Dipanjan Chowdhury, Dong Zhang, Ann Schlesinger, and Judy Lieberman
Blood, Aug 2009; 114: 1205 - 1216.
...and goat polyclonal antibodies to C23 (F-18), N-terminal and C-terminal regions of PARP-1, and PARP-2 (Santa Cruz), and PAR (Trevigen, Inc). HRP-conjugated donkey anti-goat IgG (Santa Cruz), HRP-conjugated donkey anti-rabbit and anti-mouse IgG (Amersham) were...
Requirement for Protein Synthesis at Developing Synapses
Joseph Sebeo, Kuangfu Hsiao, Ozlem Bozdagi, Dani Dumitriu, Yongchao Ge, Qiang Zhou, and Deanna L. Benson
J. Neurosci., Aug 2009; 29: 9778 - 9793.
...Sinai School of Medicine, New York, NY), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (rabbit polyclonal; 1:1000; Trevigen). Fluorescence-tagged secondary antibodies were obtained from Vector Laboratories and Jackson ImmunoResearch. For WB, species-appropriate...
Prolonged Cell Cycle Response of HeLa Cells to Low-Level Alkylation Exposure
Allen G. Schroering, Anbarasi Kothandapani, Steve M. Patrick, Saravanan Kaliyaperumal, Vishal P. Sharma, and Kandace J. Williams
Cancer Res., Aug 2009; 69: 6307 - 6314.
...The slides were then placed into neutralizing solution [0.4 mol/L Tris-HCl (pH 7.5)] for 10 min and stained with SYBR Green (Trevigen). Comets were scored using a Nikon epifluorescence microscope at 200 magnification. Fifty cells were analyzed per slide using...
SIRT1 Promotes Cell Survival under Stress by Deacetylation-Dependent Deactivation of Poly(ADP-Ribose) Polymerase 1
Senthilkumar B. Rajamohan, Vinodkumar B. Pillai, Madhu Gupta, Nagalingam R. Sundaresan, Konstantin G. Birukov, Sadhana Samant, Michael O. Hottiger, and Mahesh P. Gupta
Mol. Cell. Biol., Aug 2009; 29: 4116 - 4129.
...autoradiography or Western blotting with the appropriate antibodies. In experiments where the effect of DNA was examined, activated DNA (Trevigen) or genomic DNA (1 ug/sample) was added to the buffer before incubating PARP1 with radiolabeled NAD. Cell death assay...
Friday, September 4, 2009
Single Cell DNA Damage Assessment
FLARE-based Assessment of DNA Damage...
Trevigen’s unique FLARE™ (Fragment Length Analysis using Repair Enzymes) Assays provide the ability to detect DNA damage in single cells using a variety of DNA repair enzymes in conjunction with Trevigen’s CometAssay® single cell gel electrophoresis kit. To assess the type of DNA damage induced by a putative mutagen, drug, or treatment regimen, cells are harvested after treatment and immobilized in a layer of low melting point agarose on the FLARE Slide.
Oxidative Damage Assessment
Fpg
4040-100-FK
8-oxoguanine, DNA containing formamidopyrimidine moieties
Endonuclease III
4045-01K-FK
Thymine Glycol, 5,6-dihydrothymine, urea, 5-hydroxy-6-hydrothymine, 5,6-dihydrouracil, alloxan, 5-hydroxy-6-hydrouracil, uracil glycol, 5-hydroxy-5-methylhydantoin, 5-hydroxycytosine,5-hydroxyuracil,methyltartonylurea, thymine ring saturated or fragmentation product
hOGG1
4130-100-FK
8-oxoguanine, DNA containing formamidopyrimidine moieties
UV Damage Assessment
T4-PDG
4055-100-FK
Cis-syn isomers of cyclobutane pyrimidine dimers
cv-PDG
4065-100-FK
Cis-syn and trans-syn isomers of cyclobutane pyrimidine dimers
UVDE
4100-100-FK
Cyclobutane pyrimidine dimers, (6-4) photoproducts
Trevigen’s unique FLARE™ (Fragment Length Analysis using Repair Enzymes) Assays provide the ability to detect DNA damage in single cells using a variety of DNA repair enzymes in conjunction with Trevigen’s CometAssay® single cell gel electrophoresis kit. To assess the type of DNA damage induced by a putative mutagen, drug, or treatment regimen, cells are harvested after treatment and immobilized in a layer of low melting point agarose on the FLARE Slide.
Features
- FLARE Slides allow direct application of low melting point agarose without base layers or pretreatment for three samples
- Hydrophobic barrier allows easy application of cells directly to slide and treatment with enzyme
- Base specific DNA damage detection/enhanced damage characterization
Oxidative Damage Assessment
Fpg
4040-100-FK
8-oxoguanine, DNA containing formamidopyrimidine moieties
Endonuclease III
4045-01K-FK
Thymine Glycol, 5,6-dihydrothymine, urea, 5-hydroxy-6-hydrothymine, 5,6-dihydrouracil, alloxan, 5-hydroxy-6-hydrouracil, uracil glycol, 5-hydroxy-5-methylhydantoin, 5-hydroxycytosine,5-hydroxyuracil,methyltartonylurea, thymine ring saturated or fragmentation product
hOGG1
4130-100-FK
8-oxoguanine, DNA containing formamidopyrimidine moieties
UV Damage Assessment
T4-PDG
4055-100-FK
Cis-syn isomers of cyclobutane pyrimidine dimers
cv-PDG
4065-100-FK
Cis-syn and trans-syn isomers of cyclobutane pyrimidine dimers
UVDE
4100-100-FK
Cyclobutane pyrimidine dimers, (6-4) photoproducts
Thursday, August 20, 2009
Cultrex® Human Basement Membrane Extract
Trevigen releases human placenta derived basement membrane extract providing a pathogen and xenographic free microenvironment for human cell research. Basement Membrane Extract (BME) can be used for promotion and maintenance of a specific phenotype in a variety of cell cultures including stem cells, primary epithelial cells, primary endothelial cells and smooth muscle cells.
A basement membrane extract derived from mouse EHS sarcoma is the historical standard with which research has been done on a variety of normal and cancer cells derived from both animal and human sources. While these models do demonstrate a physiological response, there are subtle differences in the composition of the extracellular matrix as a result of tissue source and species-specificity, as it is known that there are alterations in the tumor microenvironment, and mouse-specific proteins may impact the normal human cellular response.
As an option to avoid these issues, Trevigen has developed a BME derived from normal human placenta, avoiding the presence of both tumor-derived and mouse-specific proteins in the human cell microenvironment. The human BME has been functionally tested for compatibility with human cells. The human BME has also been tested for sterility, and further PCR tested to insure the absence of eight human pathogenic viruses including Hepatitis A, B and C, HIV 1 and 2, Hantaan, Seoul and Sin Nombre.
Trevigen, Inc. is a rapidly growing biotechnology company focused on the development of products and technology for cancer research, emphasizing apoptosis, DNA damage and repair, and cancer cell function and behavior. Trevigen has been a long-standing provider of quality reagents and kits for researchers investigating programmed cell death and DNA damage and repair. A logical extension of the focus on cancer research has been the recent development of assays for cancer cell function and behavior including angiogenesis, cell invasion and tumor formation. Currently, the product portfolio contains over 500 products categorized into four processes – Apoptosis, DNA Damage and Repair, Cancer Cell Behavior, and Oxidative Stress.
Trevigen, Inc. provides a complete line of specialized proteins for cell research, cancer cell assays and recently Mesenchymal stem cell differentiation products. Visit www.trevigen.com or contact us at 1-800-873-8443 or info@trevigen.com for more information.
A basement membrane extract derived from mouse EHS sarcoma is the historical standard with which research has been done on a variety of normal and cancer cells derived from both animal and human sources. While these models do demonstrate a physiological response, there are subtle differences in the composition of the extracellular matrix as a result of tissue source and species-specificity, as it is known that there are alterations in the tumor microenvironment, and mouse-specific proteins may impact the normal human cellular response.
As an option to avoid these issues, Trevigen has developed a BME derived from normal human placenta, avoiding the presence of both tumor-derived and mouse-specific proteins in the human cell microenvironment. The human BME has been functionally tested for compatibility with human cells. The human BME has also been tested for sterility, and further PCR tested to insure the absence of eight human pathogenic viruses including Hepatitis A, B and C, HIV 1 and 2, Hantaan, Seoul and Sin Nombre.
Trevigen, Inc. is a rapidly growing biotechnology company focused on the development of products and technology for cancer research, emphasizing apoptosis, DNA damage and repair, and cancer cell function and behavior. Trevigen has been a long-standing provider of quality reagents and kits for researchers investigating programmed cell death and DNA damage and repair. A logical extension of the focus on cancer research has been the recent development of assays for cancer cell function and behavior including angiogenesis, cell invasion and tumor formation. Currently, the product portfolio contains over 500 products categorized into four processes – Apoptosis, DNA Damage and Repair, Cancer Cell Behavior, and Oxidative Stress.
Trevigen, Inc. provides a complete line of specialized proteins for cell research, cancer cell assays and recently Mesenchymal stem cell differentiation products. Visit www.trevigen.com or contact us at 1-800-873-8443 or info@trevigen.com for more information.
Friday, August 7, 2009
Recently published scientific papers citing the use of Trevigen products
Laminin-1 induces E-cadherin expression in 3-dimensional cultured breast cancer cells by inhibiting DNA methyltransferase 1 and reversing promoter methylation statusGabriel Benton, Elliott Crooke, and Jay GeorgeFASEB J, Jul 2009; 10.1096/fj.08-128702.
...dehydrogenase (GAPDH) was obtained from Trevigen (Gaithersburg, MD, USA). Secondary antibodies...
GAPDH depletion induces cell cycle arrest and resistance to antimetabolites in human carcinoma cell linesManali S Phadke, Natalia F Krynetskaia, Anurag K Mishra, and Evgeny KrynetskiyJ. Pharmacol. Exp. Ther., Jul 2009; 10.1124/jpet.109.155671.
...were detected by Neutral Comet assay using the CometAssay kit (Trevigen, Gaithersburg, MD), according to manufacturer's instructions...
...with LMA agarose (1:10 v/v), and applied on the FLARE slides (Trevigen, Gaithersburg, MD). After cell lysis in a neutral lysis buffer...
Nicked 2-glycoprotein I binds angiostatin4.5 (plasminogen kringle 1-5) and attenuates its anti-angiogenic propertyHisako Nakagawa, Shinsuke Yasuda, Eiji Matsuura, Kazuko Kobayashi, Masahiro Ieko, Hiroshi Kataoka, Tetsuya Horita, Tatsuya Atsumi, and Takao KoikeBlood, Jul 2009; 10.1182/blood-2008-12-190629.
...angiogenesis assay (DIVAA). The DIVAA assay was obtained from Trevigen Inc. (Gaithersburg, MD) and performed as previously described...
Transient dsDNA breaks during pre-replication complex assemblyEmmanouil Rampakakis and Maria Zannis-HadjopoulosNucleic Acids Res., Jul 2009; 10.1093/nar/gkp617.
...Electrophoretic mobility-shift assay (EMSA) Nuclear cell extracts (10 mug) or 100 ng of recombinant Ku (Trevigen)...
Nucleophosmin Redistribution following Heat Shock: A Role in Heat-Induced RadiosensitizationRobert P. Vanderwaal, Leonard B. Maggi, Jr., Jason D. Weber, Clayton R. Hunt, and Joseph L. Roti RotiCancer Res., Jul 2009; 10.1158/0008-5472.CAN-08-4896.
...The Neutral Comet Assay was performed using the CometAssay kit from Trevigen following manufacturer's instructions...
Estrogen Inhibits ATR Signaling to Cell Cycle Checkpoints and DNA RepairAli Pedram, Mahnaz Razandi, Albert J. Evinger, Eva Lee, and Ellis R. LevinMol. Biol. Cell, Jul 2009; 20: 3374 - 3389.
...subjected to electrophoresis. The cells were stained with DNA intercalating dye (SYBR green and visualized by epifluorescence (Trevigen, Gaithersburg, MD; UVDE Flare Assay Kit). Damaged, unrepaired DNA was seen as the tail of the Comet, quantified from 200 cells...
Regulation of transgenes in three-dimensional cultures of primary mouse mammary cells demonstrates oncogene dependence and identifies cells that survive deinductionMartin Jechlinger, Katrina Podsypanina, and Harold VarmusGenes & Dev., Jul 2009; 23: 1677 - 1688.
...Materials 3D Culture Matrix Basement Membrane Extract (Cat# 3445-048-01) and Rat Collagen I (Cat#3447-020-01) were purchased from Trevigen Inc...
The Fowlpox Virus BCL-2 Homologue, FPV039, Interacts with Activated Bax and a Discrete Subset of BH3-Only Proteins To Inhibit ApoptosisLogan Banadyga, Kirstin Veugelers, Stephanie Campbell, and Michele BarryJ. Virol., Jul 2009; 83: 7085 - 7098.
...mouse anti-Bax2D2 (1:10,000; Trevigen), mouse anti-Flag HRP (1:2,000; Sigma-Aldrich), mouse anti-Myc (clone 9E10; 1:2,500), mouse anti-T7 Tag...
Chelation of intracellular iron with the anti-fungal agent ciclopirox olamine induces cell death in leukemia and myeloma cellsYanina Eberhard, Sean McDermott, Xiaoming Wang, Marcela Gronda, Amudha Venugopal, Tabitha E. Wood, Rose Hurren, Alessandro Datti, Robert A. Batey, Jeffrey Wrana, William E. Antholine, John Dick, and Aaron D. SchimmerBlood, Jul 2009; 10.1182/blood-2009-03-209965.
...or with mouse antihuman GAPDH (Trevigen, Gaithersburg, MD). Secondary antibodies were horseradish peroxidase-conjugated...
The immune adaptor molecule SARM modulates TNF- production and microglia activation in the brainstem and restricts West Nile virus pathogenesisKristy J. Szretter, Melanie A. Samuel, Susan Gilfillan, Anja Fuchs, Marco Colonna, and Michael S. DiamondJ. Virol., Jul 2009; 10.1128/JVI.00836-09.
...TUNEL staining was performed using the NeuroTACS II In Situ Apoptosis Detection kit (Trevigen) according to the manufacturer's protocols with some modifications...
DNA Damage-Induced Phosphorylation of TRF2 Is Required for the Fast Pathway of DNA Double-Strand Break RepairNazmul Huda, Hiromi Tanaka, Marc S. Mendonca, and David GilleyMol. Cell. Biol., Jul 2009; 29: 3597 - 3604.
...To study DNA DSB repair, neutral comet assays were performed using CometAssaykits (Trevigen). Induced and uninduced cells were prepared and exposed to X rays at 20 Gy on ice...
Zip3 (Slc39a3) functions in zinc reuptake from the alveolar lumen in lactating mammary glandShannon L. Kelleher, Veronica Lopez, Bo Lönnerdal, Jodi Dufner-Beattie, and Glen K. AndrewsAm J Physiol Regulatory Integrative Comp Physiol, Jul 2009; 297: R194 - R201.
...apoptotic cells in the mammary gland was assessed in situ using the TACS-XL Basic In Situ Apoptosis (TUNEL) Detection kit (Trevigen, Gaithersburg, MD) per the manufacturers instructions...
...dehydrogenase (GAPDH) was obtained from Trevigen (Gaithersburg, MD, USA). Secondary antibodies...
GAPDH depletion induces cell cycle arrest and resistance to antimetabolites in human carcinoma cell linesManali S Phadke, Natalia F Krynetskaia, Anurag K Mishra, and Evgeny KrynetskiyJ. Pharmacol. Exp. Ther., Jul 2009; 10.1124/jpet.109.155671.
...were detected by Neutral Comet assay using the CometAssay kit (Trevigen, Gaithersburg, MD), according to manufacturer's instructions...
...with LMA agarose (1:10 v/v), and applied on the FLARE slides (Trevigen, Gaithersburg, MD). After cell lysis in a neutral lysis buffer...
Nicked 2-glycoprotein I binds angiostatin4.5 (plasminogen kringle 1-5) and attenuates its anti-angiogenic propertyHisako Nakagawa, Shinsuke Yasuda, Eiji Matsuura, Kazuko Kobayashi, Masahiro Ieko, Hiroshi Kataoka, Tetsuya Horita, Tatsuya Atsumi, and Takao KoikeBlood, Jul 2009; 10.1182/blood-2008-12-190629.
...angiogenesis assay (DIVAA). The DIVAA assay was obtained from Trevigen Inc. (Gaithersburg, MD) and performed as previously described...
Transient dsDNA breaks during pre-replication complex assemblyEmmanouil Rampakakis and Maria Zannis-HadjopoulosNucleic Acids Res., Jul 2009; 10.1093/nar/gkp617.
...Electrophoretic mobility-shift assay (EMSA) Nuclear cell extracts (10 mug) or 100 ng of recombinant Ku (Trevigen)...
Nucleophosmin Redistribution following Heat Shock: A Role in Heat-Induced RadiosensitizationRobert P. Vanderwaal, Leonard B. Maggi, Jr., Jason D. Weber, Clayton R. Hunt, and Joseph L. Roti RotiCancer Res., Jul 2009; 10.1158/0008-5472.CAN-08-4896.
...The Neutral Comet Assay was performed using the CometAssay kit from Trevigen following manufacturer's instructions...
Estrogen Inhibits ATR Signaling to Cell Cycle Checkpoints and DNA RepairAli Pedram, Mahnaz Razandi, Albert J. Evinger, Eva Lee, and Ellis R. LevinMol. Biol. Cell, Jul 2009; 20: 3374 - 3389.
...subjected to electrophoresis. The cells were stained with DNA intercalating dye (SYBR green and visualized by epifluorescence (Trevigen, Gaithersburg, MD; UVDE Flare Assay Kit). Damaged, unrepaired DNA was seen as the tail of the Comet, quantified from 200 cells...
Regulation of transgenes in three-dimensional cultures of primary mouse mammary cells demonstrates oncogene dependence and identifies cells that survive deinductionMartin Jechlinger, Katrina Podsypanina, and Harold VarmusGenes & Dev., Jul 2009; 23: 1677 - 1688.
...Materials 3D Culture Matrix Basement Membrane Extract (Cat# 3445-048-01) and Rat Collagen I (Cat#3447-020-01) were purchased from Trevigen Inc...
The Fowlpox Virus BCL-2 Homologue, FPV039, Interacts with Activated Bax and a Discrete Subset of BH3-Only Proteins To Inhibit ApoptosisLogan Banadyga, Kirstin Veugelers, Stephanie Campbell, and Michele BarryJ. Virol., Jul 2009; 83: 7085 - 7098.
...mouse anti-Bax2D2 (1:10,000; Trevigen), mouse anti-Flag HRP (1:2,000; Sigma-Aldrich), mouse anti-Myc (clone 9E10; 1:2,500), mouse anti-T7 Tag...
Chelation of intracellular iron with the anti-fungal agent ciclopirox olamine induces cell death in leukemia and myeloma cellsYanina Eberhard, Sean McDermott, Xiaoming Wang, Marcela Gronda, Amudha Venugopal, Tabitha E. Wood, Rose Hurren, Alessandro Datti, Robert A. Batey, Jeffrey Wrana, William E. Antholine, John Dick, and Aaron D. SchimmerBlood, Jul 2009; 10.1182/blood-2009-03-209965.
...or with mouse antihuman GAPDH (Trevigen, Gaithersburg, MD). Secondary antibodies were horseradish peroxidase-conjugated...
The immune adaptor molecule SARM modulates TNF- production and microglia activation in the brainstem and restricts West Nile virus pathogenesisKristy J. Szretter, Melanie A. Samuel, Susan Gilfillan, Anja Fuchs, Marco Colonna, and Michael S. DiamondJ. Virol., Jul 2009; 10.1128/JVI.00836-09.
...TUNEL staining was performed using the NeuroTACS II In Situ Apoptosis Detection kit (Trevigen) according to the manufacturer's protocols with some modifications...
DNA Damage-Induced Phosphorylation of TRF2 Is Required for the Fast Pathway of DNA Double-Strand Break RepairNazmul Huda, Hiromi Tanaka, Marc S. Mendonca, and David GilleyMol. Cell. Biol., Jul 2009; 29: 3597 - 3604.
...To study DNA DSB repair, neutral comet assays were performed using CometAssaykits (Trevigen). Induced and uninduced cells were prepared and exposed to X rays at 20 Gy on ice...
Zip3 (Slc39a3) functions in zinc reuptake from the alveolar lumen in lactating mammary glandShannon L. Kelleher, Veronica Lopez, Bo Lönnerdal, Jodi Dufner-Beattie, and Glen K. AndrewsAm J Physiol Regulatory Integrative Comp Physiol, Jul 2009; 297: R194 - R201.
...apoptotic cells in the mammary gland was assessed in situ using the TACS-XL Basic In Situ Apoptosis (TUNEL) Detection kit (Trevigen, Gaithersburg, MD) per the manufacturers instructions...
Thursday, July 30, 2009
Kits for Stem Cell Research
Mesenchymal Stem Cells (MSC), also known as marrow stromal cells, are a self renewing population of multipotent cells present in bone marrow and many other adult tissues. MSC can be isolated from bone marrow by adherence to plastic and can differentiate into multiple lineage-specific cells that form bone, fat, cartilage, muscle, and tendon.
Mesenchymal Stem Cell Starter Kit (cat# 5000-001-K)
Kit includes primary Mesenchymal Stem Cells isolated and purified from Rat Bone Marrow plus qualified medium and serum to support undifferentiated growth.
Mesenchymal Stem Cell Adipogenic Differentiation Kit (cat# 5010-024-K)
Kit includes all the reagents necessary for differentiating Mesenchymal Stem Cells into Adipocytes.
Mesenchymal Stem Cell Osteogenic Differentiation Kit (cat# 5011-024-K)
Kit includes all the reagents necessary for differentiating Mesenchymal Stem Cells into Osteoblasts.
Rat Mesenchymal Stem Cell Replenisher Kit (cat# 5000-001-R)
Kit includes qualified medium and serum to support undifferentiated growth of Rat Mesenchymal Stem Cells.
Mesenchymal Stem Cell Starter Kit (cat# 5000-001-K)
Kit includes primary Mesenchymal Stem Cells isolated and purified from Rat Bone Marrow plus qualified medium and serum to support undifferentiated growth.
Mesenchymal Stem Cell Adipogenic Differentiation Kit (cat# 5010-024-K)
Kit includes all the reagents necessary for differentiating Mesenchymal Stem Cells into Adipocytes.
Mesenchymal Stem Cell Osteogenic Differentiation Kit (cat# 5011-024-K)
Kit includes all the reagents necessary for differentiating Mesenchymal Stem Cells into Osteoblasts.
Rat Mesenchymal Stem Cell Replenisher Kit (cat# 5000-001-R)
Kit includes qualified medium and serum to support undifferentiated growth of Rat Mesenchymal Stem Cells.
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