New Citations using Trevigen Products

The Recombination Protein RAD52 Cooperates with the Excision Repair Protein OGG1 for the Repair of Oxidative Lesions in Mammalian Cells
Nadja C. de Souza-Pinto, Scott Maynard, Kazunari Hashiguchi, Jingping Hu, Meltem Muftuoglu, and Vilhelm A. Bohr
Mol. Cell. Biol., Aug 2009; 29: 4441 - 4454.
...incubated in primary anti-8-oxoG antibody (Trevigen) at a 1:250 dilution in 10 goat serum-PBS...heterochromatic DNA (7, 32, 40; see also the Trevigen web site). For the control, the results...8-oxoG antibody (Fig. 7C) that was used (Trevigen) in two ways. First, treatment of HeLa...

Poly(ADP-ribose) polymerase inhibitor ABT-888 potentiates the cytotoxic activity of temozolomide in leukemia cells: influence of mismatch repair status and O6-methylguanine-DNA methyltransferase activity
Terzah M. Horton, Gaye Jenkins, Debananda Pati, Linna Zhang, M. Eileen Dolan, Albert Ribes-Zamora, Alison A. Bertuch, Susan M. Blaney, Shannon L. Delaney, Madhuri Hegde, and Stacey L. Berg
Mol. Cancer Ther., Aug 2009; 8: 2232 - 2242.
...a commercially available PARP assay (Trevigen). Cell lysates were prepared from 1 107 leukemia cells using 1 PARP buffer (Trevigen) supplemented with 0.4 mmol/L phenylmethylsulfonyl...in triplicate, and recombinant PARP (Trevigen) was used to produce a standard curve...

Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler
Aaron J. Gottschalk, Gyula Timinszky, Stephanie E. Kong, Jingji Jin, Yong Cai, Selene K. Swanson, Michael P. Washburn, Laurence Florens, Andreas G. Ladurner, Joan W. Conaway, and Ronald C. Conaway
PNAS, Aug 2009; 106: 13770 - 13774.
...pmol) Flag-Alc1 (wild-type, E175Q, or D723A) from HEK293/FRT cells or SF9 cells, 115 ng (1 pmol) Parp1 (Trevigen), recombinant Parg (Trevigen), 34 muM nicotine adenine dinucleotide, and 150 ng mono- or oligonucleosomes from HeLa cells (37...

Differences in Immunolocalization of Kim-1, RPA-1, and RPA-2 in Kidneys of Gentamicin-, Cisplatin-, and Valproic Acid-Treated Rats: Potential Role of iNOS and Nitrotyrosine
Jun Zhang, Peter L. Goering, Parvaneh Espandiari, Martin Shaw, Joseph V. Bonventre, Vishal S. Vaidya, Ronald P. Brown, Joe Keenan, Cormac G. Kilty, Nakissa Sadrieh, and Joseph P. Hanig
Toxicol Pathol, Aug 2009; 37: 629 - 643.
...was detected using the TACS in situ apoptotic detection kit (Trevigen, Inc., Gaithersburg, MD). The TUNEL procedures were as follows...ten minutes. In the final step, uncolored soluble chromogen (Trevigen blue label) was enzymatically converted into an insoluble blue-colored...

Dietary zinc restriction and repletion affects DNA integrity in healthy men
Yang Song, Carolyn S Chung, Richard S Bruno, Maret G Traber, Kenneth H Brown, Janet C King, and Emily Ho
Am. J. Clinical Nutrition, Aug 2009; 90: 321 - 328.
...mixed with 200 muL 0.5% low-melting-point agarose and applied onto microscope slides (Trevigen, Gaithersburg, MD). Slides were stored in lysis buffer (Trevigen, Gaithersburg, MD) and shipped to Oregon State University within 3 d. At Oregon State...

1-Adrenergic Receptors Regulate Neurogenesis and Gliogenesis
Manveen K. Gupta, Robert S. Papay, Chris W. D. Jurgens, Robert J. Gaivin, Ting Shi, Van A. Doze, and Dianne M. Perez
Mol. Pharmacol., Aug 2009; 76: 314 - 326.
...detection. Apoptosis Assay. Neurospheres were grown and analyzed for apoptosis by using the FlowTACS Apoptosis Detection Kit (Trevigen, Gaithersburg, MD). Terminal deoxynucleotidyl transferase end-labeling of the free 3-hydroxyl residues in the fragmented DNA...

PP2A-dependent Dephosphorylation of Replication Protein A (RPA) is Required for the Repair of DNA breaks Induced by Replication Stress
Junjie Feng, Timothy Wakeman, Sheila Yong, Xiaohua Wu, Sally Kornbluth, and Xiao-Fan Wang
Mol. Cell. Biol., Aug 2009; 10.1128/MCB.00191-09.
...repair kinetics of HU-induced DNA breaks was evaluated by the alkaline comet2 assay according to the manufacturer's protocol (Trevigen). Briefly, cells were pulse-3 exposed to HU (0.2 mM, 24 hr) and harvested at various recovery time points for single-4 cell...

CD133+ Glioblastoma Stem-like Cells are Radiosensitive with a Defective DNA Damage Response Compared with Established Cell Lines
Amy M. McCord, Muhammad Jamal, Eli S. Williams, Kevin Camphausen, and Philip J. Tofilon
Clin. Cancer Res., Aug 2009; 15: 5145 - 5153.
...the neutral comet assay was done using a commercially available kit according to the recommendations from the manufacturer (Trevigen) with slight modifications. Briefly, monolayer cultures were irradiated (10 Gy), and at specified times, single-cell suspensions...

Nucleophosmin Redistribution following Heat Shock: A Role in Heat-Induced Radiosensitization
Robert P. Vanderwaal, Leonard B. Maggi, Jr., Jason D. Weber, Clayton R. Hunt, and Joseph L. Roti Roti
Cancer Res., Aug 2009; 69: 6454 - 6462.
...on an Olympus fluorescent microscope. Assay for DSBs. The Neutral Comet Assay was performed using the Comet Assay kit from Trevigen following manufacturers instructions. Proteinase K (Sigma), when used, was added to the lysis solution (0.27 U/mL). Lysis occurred...

A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases
Dorjbal Dorjsuren, David M. Wilson, III, William A. Beard, John P. McDonald, Christopher P. Austin, Roger Woodgate, Samuel H. Wilson, and Anton Simeonov
Nucleic Acids Res., Aug 2009; 10.1093/nar/gkp641.
...plates following previously described protocols (27). Enzymes Recombinant human DNA pol beta was either purchased from Trevigen Inc. (Gaithersburg, MD) or produced via published protocols (28). Equivalent performance was noted for the enzyme obtained...

Modulation of Carcinogen Metabolism by Nitric Oxide-Aspirin 2 Is Associated with Suppression of DNA Damage and DNA Adduct Formation
Christopher J. MacDonald, Robert Y. S. Cheng, David D. Roberts, David A. Wink, and Grace Chao Yeh
J. Biol. Chem., Aug 2009; 284: 22099 - 22107.
...Single cell gel electrophoresis assay (31) with alkaline electrophoresis was performed according to the CometAssay protocol (Trevigen, Gaithersburg, MD), and under protective yellow lighting. In brief, HepG2 cells were treated with either 10 mum BP or 10 nm...

Synergistic effects of autologous cell and hepatocyte growth factor gene therapy for neovascularization in the murine model of hindlimb ischemia.
Yasutaka Yamamoto, Takashi Matsuura, Genta Narazaki, Miyoko Sugitani, Kohei Tanaka, Akihiro Maeda, Goshi Shiota, Kenzo Sato, Akio Yoshida, and Ichiro Hisatome
Am J Physiol Heart Circ Physiol, Aug 2009; 10.1152/ajpheart.00321.2009.
...terminal deoxynucleotide168 transferase-mediated dUTP nick end labeling (TUNEL) using a commercially169 available kit (TACS/XL, Trevigen, Gaithersburg, MD).170 171 Migration assay172 12 A total of 4 ? 105 aMNC in 0.5 mL of DMEM with 0.4% FBS were seeded173 in...

The cytotoxic T lymphocyte protease granzyme A cleaves and inactivates poly(adenosine 5'-diphosphate-ribose) polymerase-1
Pengcheng Zhu, Denis Martinvalet, Dipanjan Chowdhury, Dong Zhang, Ann Schlesinger, and Judy Lieberman
Blood, Aug 2009; 114: 1205 - 1216.
...and goat polyclonal antibodies to C23 (F-18), N-terminal and C-terminal regions of PARP-1, and PARP-2 (Santa Cruz), and PAR (Trevigen, Inc). HRP-conjugated donkey anti-goat IgG (Santa Cruz), HRP-conjugated donkey anti-rabbit and anti-mouse IgG (Amersham) were...

Requirement for Protein Synthesis at Developing Synapses
Joseph Sebeo, Kuangfu Hsiao, Ozlem Bozdagi, Dani Dumitriu, Yongchao Ge, Qiang Zhou, and Deanna L. Benson
J. Neurosci., Aug 2009; 29: 9778 - 9793.
...Sinai School of Medicine, New York, NY), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (rabbit polyclonal; 1:1000; Trevigen). Fluorescence-tagged secondary antibodies were obtained from Vector Laboratories and Jackson ImmunoResearch. For WB, species-appropriate...

Prolonged Cell Cycle Response of HeLa Cells to Low-Level Alkylation Exposure
Allen G. Schroering, Anbarasi Kothandapani, Steve M. Patrick, Saravanan Kaliyaperumal, Vishal P. Sharma, and Kandace J. Williams
Cancer Res., Aug 2009; 69: 6307 - 6314.
...The slides were then placed into neutralizing solution [0.4 mol/L Tris-HCl (pH 7.5)] for 10 min and stained with SYBR Green (Trevigen). Comets were scored using a Nikon epifluorescence microscope at 200 magnification. Fifty cells were analyzed per slide using...

SIRT1 Promotes Cell Survival under Stress by Deacetylation-Dependent Deactivation of Poly(ADP-Ribose) Polymerase 1
Senthilkumar B. Rajamohan, Vinodkumar B. Pillai, Madhu Gupta, Nagalingam R. Sundaresan, Konstantin G. Birukov, Sadhana Samant, Michael O. Hottiger, and Mahesh P. Gupta
Mol. Cell. Biol., Aug 2009; 29: 4116 - 4129.
...autoradiography or Western blotting with the appropriate antibodies. In experiments where the effect of DNA was examined, activated DNA (Trevigen) or genomic DNA (1 ug/sample) was added to the buffer before incubating PARP1 with radiolabeled NAD. Cell death assay...

Single Cell DNA Damage Assessment

FLARE-based Assessment of DNA Damage...
Trevigen’s unique FLARE™ (Fragment Length Analysis using Repair Enzymes) Assays provide the ability to detect DNA damage in single cells using a variety of DNA repair enzymes in conjunction with Trevigen’s CometAssay® single cell gel electrophoresis kit. To assess the type of DNA damage induced by a putative mutagen, drug, or treatment regimen, cells are harvested after treatment and immobilized in a layer of low melting point agarose on the FLARE Slide.

Features

• FLARE Slides allow direct application of low melting point agarose without base layers or pretreatment for three samples
• Hydrophobic barrier allows easy application of cells directly to slide and treatment with enzyme
• Base specific DNA damage detection/enhanced damage characterization

Oxidative Damage Assessment
Fpg
4040-100-FK
8-oxoguanine, DNA containing formamidopyrimidine moieties

Endonuclease III
4045-01K-FK
Thymine Glycol, 5,6-dihydrothymine, urea, 5-hydroxy-6-hydrothymine, 5,6-dihydrouracil, alloxan, 5-hydroxy-6-hydrouracil, uracil glycol, 5-hydroxy-5-methylhydantoin, 5-hydroxycytosine,5-hydroxyuracil,methyltartonylurea, thymine ring saturated or fragmentation product

hOGG1
4130-100-FK
8-oxoguanine, DNA containing formamidopyrimidine moieties

UV Damage Assessment
T4-PDG
4055-100-FK
Cis-syn isomers of cyclobutane pyrimidine dimers

cv-PDG
4065-100-FK
Cis-syn and trans-syn isomers of cyclobutane pyrimidine dimers

UVDE
4100-100-FK
Cyclobutane pyrimidine dimers, (6-4) photoproducts

Cultrex® Human Basement Membrane Extract

Trevigen releases human placenta derived basement membrane extract providing a pathogen and xenographic free microenvironment for human cell research. Basement Membrane Extract (BME) can be used for promotion and maintenance of a specific phenotype in a variety of cell cultures including stem cells, primary epithelial cells, primary endothelial cells and smooth muscle cells.

A basement membrane extract derived from mouse EHS sarcoma is the historical standard with which research has been done on a variety of normal and cancer cells derived from both animal and human sources. While these models do demonstrate a physiological response, there are subtle differences in the composition of the extracellular matrix as a result of tissue source and species-specificity, as it is known that there are alterations in the tumor microenvironment, and mouse-specific proteins may impact the normal human cellular response.

As an option to avoid these issues, Trevigen has developed a BME derived from normal human placenta, avoiding the presence of both tumor-derived and mouse-specific proteins in the human cell microenvironment. The human BME has been functionally tested for compatibility with human cells. The human BME has also been tested for sterility, and further PCR tested to insure the absence of eight human pathogenic viruses including Hepatitis A, B and C, HIV 1 and 2, Hantaan, Seoul and Sin Nombre.

Trevigen, Inc. is a rapidly growing biotechnology company focused on the development of products and technology for cancer research, emphasizing apoptosis, DNA damage and repair, and cancer cell function and behavior. Trevigen has been a long-standing provider of quality reagents and kits for researchers investigating programmed cell death and DNA damage and repair. A logical extension of the focus on cancer research has been the recent development of assays for cancer cell function and behavior including angiogenesis, cell invasion and tumor formation. Currently, the product portfolio contains over 500 products categorized into four processes – Apoptosis, DNA Damage and Repair, Cancer Cell Behavior, and Oxidative Stress.

Trevigen, Inc. provides a complete line of specialized proteins for cell research, cancer cell assays and recently Mesenchymal stem cell differentiation products. Visit www.trevigen.com or contact us at 1-800-873-8443 or info@trevigen.com for more information.

Recently published scientific papers citing the use of Trevigen products

Laminin-1 induces E-cadherin expression in 3-dimensional cultured breast cancer cells by inhibiting DNA methyltransferase 1 and reversing promoter methylation statusGabriel Benton, Elliott Crooke, and Jay GeorgeFASEB J, Jul 2009; 10.1096/fj.08-128702.
...dehydrogenase (GAPDH) was obtained from Trevigen (Gaithersburg, MD, USA). Secondary antibodies...

GAPDH depletion induces cell cycle arrest and resistance to antimetabolites in human carcinoma cell linesManali S Phadke, Natalia F Krynetskaia, Anurag K Mishra, and Evgeny KrynetskiyJ. Pharmacol. Exp. Ther., Jul 2009; 10.1124/jpet.109.155671.
...were detected by Neutral Comet assay using the CometAssay kit (Trevigen, Gaithersburg, MD), according to manufacturer's instructions...
...with LMA agarose (1:10 v/v), and applied on the FLARE slides (Trevigen, Gaithersburg, MD). After cell lysis in a neutral lysis buffer...

Nicked 2-glycoprotein I binds angiostatin4.5 (plasminogen kringle 1-5) and attenuates its anti-angiogenic propertyHisako Nakagawa, Shinsuke Yasuda, Eiji Matsuura, Kazuko Kobayashi, Masahiro Ieko, Hiroshi Kataoka, Tetsuya Horita, Tatsuya Atsumi, and Takao KoikeBlood, Jul 2009; 10.1182/blood-2008-12-190629.
...angiogenesis assay (DIVAA). The DIVAA assay was obtained from Trevigen Inc. (Gaithersburg, MD) and performed as previously described...

Transient dsDNA breaks during pre-replication complex assemblyEmmanouil Rampakakis and Maria Zannis-HadjopoulosNucleic Acids Res., Jul 2009; 10.1093/nar/gkp617.
...Electrophoretic mobility-shift assay (EMSA) Nuclear cell extracts (10 mug) or 100 ng of recombinant Ku (Trevigen)...

Nucleophosmin Redistribution following Heat Shock: A Role in Heat-Induced RadiosensitizationRobert P. Vanderwaal, Leonard B. Maggi, Jr., Jason D. Weber, Clayton R. Hunt, and Joseph L. Roti RotiCancer Res., Jul 2009; 10.1158/0008-5472.CAN-08-4896.
...The Neutral Comet Assay was performed using the CometAssay kit from Trevigen following manufacturer's instructions...

Estrogen Inhibits ATR Signaling to Cell Cycle Checkpoints and DNA RepairAli Pedram, Mahnaz Razandi, Albert J. Evinger, Eva Lee, and Ellis R. LevinMol. Biol. Cell, Jul 2009; 20: 3374 - 3389.
...subjected to electrophoresis. The cells were stained with DNA intercalating dye (SYBR green and visualized by epifluorescence (Trevigen, Gaithersburg, MD; UVDE Flare Assay Kit). Damaged, unrepaired DNA was seen as the tail of the Comet, quantified from 200 cells...

Regulation of transgenes in three-dimensional cultures of primary mouse mammary cells demonstrates oncogene dependence and identifies cells that survive deinductionMartin Jechlinger, Katrina Podsypanina, and Harold VarmusGenes & Dev., Jul 2009; 23: 1677 - 1688.
...Materials 3D Culture Matrix Basement Membrane Extract (Cat# 3445-048-01) and Rat Collagen I (Cat#3447-020-01) were purchased from Trevigen Inc...

The Fowlpox Virus BCL-2 Homologue, FPV039, Interacts with Activated Bax and a Discrete Subset of BH3-Only Proteins To Inhibit ApoptosisLogan Banadyga, Kirstin Veugelers, Stephanie Campbell, and Michele BarryJ. Virol., Jul 2009; 83: 7085 - 7098.
...mouse anti-Bax2D2 (1:10,000; Trevigen), mouse anti-Flag HRP (1:2,000; Sigma-Aldrich), mouse anti-Myc (clone 9E10; 1:2,500), mouse anti-T7 Tag...

Chelation of intracellular iron with the anti-fungal agent ciclopirox olamine induces cell death in leukemia and myeloma cellsYanina Eberhard, Sean McDermott, Xiaoming Wang, Marcela Gronda, Amudha Venugopal, Tabitha E. Wood, Rose Hurren, Alessandro Datti, Robert A. Batey, Jeffrey Wrana, William E. Antholine, John Dick, and Aaron D. SchimmerBlood, Jul 2009; 10.1182/blood-2009-03-209965.
...or with mouse antihuman GAPDH (Trevigen, Gaithersburg, MD). Secondary antibodies were horseradish peroxidase-conjugated...

The immune adaptor molecule SARM modulates TNF- production and microglia activation in the brainstem and restricts West Nile virus pathogenesisKristy J. Szretter, Melanie A. Samuel, Susan Gilfillan, Anja Fuchs, Marco Colonna, and Michael S. DiamondJ. Virol., Jul 2009; 10.1128/JVI.00836-09.
...TUNEL staining was performed using the NeuroTACS II In Situ Apoptosis Detection kit (Trevigen) according to the manufacturer's protocols with some modifications...

DNA Damage-Induced Phosphorylation of TRF2 Is Required for the Fast Pathway of DNA Double-Strand Break RepairNazmul Huda, Hiromi Tanaka, Marc S. Mendonca, and David GilleyMol. Cell. Biol., Jul 2009; 29: 3597 - 3604.
...To study DNA DSB repair, neutral comet assays were performed using CometAssaykits (Trevigen). Induced and uninduced cells were prepared and exposed to X rays at 20 Gy on ice...

Zip3 (Slc39a3) functions in zinc reuptake from the alveolar lumen in lactating mammary glandShannon L. Kelleher, Veronica Lopez, Bo Lönnerdal, Jodi Dufner-Beattie, and Glen K. AndrewsAm J Physiol Regulatory Integrative Comp Physiol, Jul 2009; 297: R194 - R201.
...apoptotic cells in the mammary gland was assessed in situ using the TACS-XL Basic In Situ Apoptosis (TUNEL) Detection kit (Trevigen, Gaithersburg, MD) per the manufacturers instructions...

Kits for Stem Cell Research

Mesenchymal Stem Cells (MSC), also known as marrow stromal cells, are a self renewing population of multipotent cells present in bone marrow and many other adult tissues. MSC can be isolated from bone marrow by adherence to plastic and can differentiate into multiple lineage-specific cells that form bone, fat, cartilage, muscle, and tendon.

Mesenchymal Stem Cell Starter Kit (cat# 5000-001-K)
Kit includes primary Mesenchymal Stem Cells isolated and purified from Rat Bone Marrow plus qualified medium and serum to support undifferentiated growth.

Mesenchymal Stem Cell Adipogenic Differentiation Kit (cat# 5010-024-K)
Kit includes all the reagents necessary for differentiating Mesenchymal Stem Cells into Adipocytes.

Mesenchymal Stem Cell Osteogenic Differentiation Kit (cat# 5011-024-K)
Kit includes all the reagents necessary for differentiating Mesenchymal Stem Cells into Osteoblasts.

Rat Mesenchymal Stem Cell Replenisher Kit (cat# 5000-001-R)
Kit includes qualified medium and serum to support undifferentiated growth of Rat Mesenchymal Stem Cells.