Laminin-1 induces E-cadherin expression in 3-dimensional cultured breast cancer cells by inhibiting DNA methyltransferase 1 and reversing promoter methylation statusGabriel Benton, Elliott Crooke, and Jay GeorgeFASEB J, Jul 2009; 10.1096/fj.08-128702.
...dehydrogenase (GAPDH) was obtained from Trevigen (Gaithersburg, MD, USA). Secondary antibodies...
GAPDH depletion induces cell cycle arrest and resistance to antimetabolites in human carcinoma cell linesManali S Phadke, Natalia F Krynetskaia, Anurag K Mishra, and Evgeny KrynetskiyJ. Pharmacol. Exp. Ther., Jul 2009; 10.1124/jpet.109.155671.
...were detected by Neutral Comet assay using the CometAssay kit (Trevigen, Gaithersburg, MD), according to manufacturer's instructions...
...with LMA agarose (1:10 v/v), and applied on the FLARE slides (Trevigen, Gaithersburg, MD). After cell lysis in a neutral lysis buffer...
Nicked 2-glycoprotein I binds angiostatin4.5 (plasminogen kringle 1-5) and attenuates its anti-angiogenic propertyHisako Nakagawa, Shinsuke Yasuda, Eiji Matsuura, Kazuko Kobayashi, Masahiro Ieko, Hiroshi Kataoka, Tetsuya Horita, Tatsuya Atsumi, and Takao KoikeBlood, Jul 2009; 10.1182/blood-2008-12-190629.
...angiogenesis assay (DIVAA). The DIVAA assay was obtained from Trevigen Inc. (Gaithersburg, MD) and performed as previously described...
Transient dsDNA breaks during pre-replication complex assemblyEmmanouil Rampakakis and Maria Zannis-HadjopoulosNucleic Acids Res., Jul 2009; 10.1093/nar/gkp617.
...Electrophoretic mobility-shift assay (EMSA) Nuclear cell extracts (10 mug) or 100 ng of recombinant Ku (Trevigen)...
Nucleophosmin Redistribution following Heat Shock: A Role in Heat-Induced RadiosensitizationRobert P. Vanderwaal, Leonard B. Maggi, Jr., Jason D. Weber, Clayton R. Hunt, and Joseph L. Roti RotiCancer Res., Jul 2009; 10.1158/0008-5472.CAN-08-4896.
...The Neutral Comet Assay was performed using the CometAssay kit from Trevigen following manufacturer's instructions...
Estrogen Inhibits ATR Signaling to Cell Cycle Checkpoints and DNA RepairAli Pedram, Mahnaz Razandi, Albert J. Evinger, Eva Lee, and Ellis R. LevinMol. Biol. Cell, Jul 2009; 20: 3374 - 3389.
...subjected to electrophoresis. The cells were stained with DNA intercalating dye (SYBR green and visualized by epifluorescence (Trevigen, Gaithersburg, MD; UVDE Flare Assay Kit). Damaged, unrepaired DNA was seen as the tail of the Comet, quantified from 200 cells...
Regulation of transgenes in three-dimensional cultures of primary mouse mammary cells demonstrates oncogene dependence and identifies cells that survive deinductionMartin Jechlinger, Katrina Podsypanina, and Harold VarmusGenes & Dev., Jul 2009; 23: 1677 - 1688.
...Materials 3D Culture Matrix Basement Membrane Extract (Cat# 3445-048-01) and Rat Collagen I (Cat#3447-020-01) were purchased from Trevigen Inc...
The Fowlpox Virus BCL-2 Homologue, FPV039, Interacts with Activated Bax and a Discrete Subset of BH3-Only Proteins To Inhibit ApoptosisLogan Banadyga, Kirstin Veugelers, Stephanie Campbell, and Michele BarryJ. Virol., Jul 2009; 83: 7085 - 7098.
...mouse anti-Bax2D2 (1:10,000; Trevigen), mouse anti-Flag HRP (1:2,000; Sigma-Aldrich), mouse anti-Myc (clone 9E10; 1:2,500), mouse anti-T7 Tag...
Chelation of intracellular iron with the anti-fungal agent ciclopirox olamine induces cell death in leukemia and myeloma cellsYanina Eberhard, Sean McDermott, Xiaoming Wang, Marcela Gronda, Amudha Venugopal, Tabitha E. Wood, Rose Hurren, Alessandro Datti, Robert A. Batey, Jeffrey Wrana, William E. Antholine, John Dick, and Aaron D. SchimmerBlood, Jul 2009; 10.1182/blood-2009-03-209965.
...or with mouse antihuman GAPDH (Trevigen, Gaithersburg, MD). Secondary antibodies were horseradish peroxidase-conjugated...
The immune adaptor molecule SARM modulates TNF- production and microglia activation in the brainstem and restricts West Nile virus pathogenesisKristy J. Szretter, Melanie A. Samuel, Susan Gilfillan, Anja Fuchs, Marco Colonna, and Michael S. DiamondJ. Virol., Jul 2009; 10.1128/JVI.00836-09.
...TUNEL staining was performed using the NeuroTACS II In Situ Apoptosis Detection kit (Trevigen) according to the manufacturer's protocols with some modifications...
DNA Damage-Induced Phosphorylation of TRF2 Is Required for the Fast Pathway of DNA Double-Strand Break RepairNazmul Huda, Hiromi Tanaka, Marc S. Mendonca, and David GilleyMol. Cell. Biol., Jul 2009; 29: 3597 - 3604.
...To study DNA DSB repair, neutral comet assays were performed using CometAssaykits (Trevigen). Induced and uninduced cells were prepared and exposed to X rays at 20 Gy on ice...
Zip3 (Slc39a3) functions in zinc reuptake from the alveolar lumen in lactating mammary glandShannon L. Kelleher, Veronica Lopez, Bo Lönnerdal, Jodi Dufner-Beattie, and Glen K. AndrewsAm J Physiol Regulatory Integrative Comp Physiol, Jul 2009; 297: R194 - R201.
...apoptotic cells in the mammary gland was assessed in situ using the TACS-XL Basic In Situ Apoptosis (TUNEL) Detection kit (Trevigen, Gaithersburg, MD) per the manufacturers instructions...
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